Application | Comment | Organism |
---|---|---|
medicine | inhaled DNase I into the airways reduces viscoelasticity of sputum, improves lung function of cystic fibrosis patients | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in human 293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Q9R/E13R/N74K | most hyperactive variant, 35fold more active than wild type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | 50% inhibition at 60 mM, mutated enzyme less sensitive against increased salt concentrations | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation synergistic with Mg2+ | Homo sapiens | |
Mg2+ | activation synergistic with Ca2+ | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
190mer DNA fragment + H2O | - |
Homo sapiens | 5'-phosphooligonucleotides + ? | - |
? | |
linearized plasmid-DNA + H2O | - |
Homo sapiens | 5'-phosphooligonucleotides + H2O | - |
? | |
p-nitrophenyl phenylphosphonate + H2O | - |
Homo sapiens | p-nitrophenol + phenylphosphoric acid | - |
? | |
supercoiled plasmid DNA + H2O | - |
Homo sapiens | linear DNA + ? | formation of a relaxed circle that subsequently converts to linear DNA if it is nicked on the other strand at the same position | ? |