Cloned (Comment) | Organism |
---|---|
deducing amino acid composition from cDNA | Oreochromis mossambicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetate | 50% inhibition at 0.1 M in presence of 4 mM CuCl2 after 15 min | Oreochromis mossambicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | highest activity at 10 mM, doubled activity upon addition of 1 mM Ca2+ | Oreochromis mossambicus | |
Mn2+ | highest activity at 1 mM, addition of Ca2+ doesn't change activity | Oreochromis mossambicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration, SDS-PAGE, thin-layer IEF, MS analysis | Oreochromis mossambicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | Oreochromis mossambicus | - |
5'-phosphooligonucleotides + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oreochromis mossambicus | - |
tilapia fish | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | carbohydrate-chain at Asn 18 | Oreochromis mossambicus |
Purification (Comment) | Organism |
---|---|
to homogeneity by 4step chromatography | Oreochromis mossambicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatopancreas | - |
Oreochromis mossambicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
units defined as absorbance at 260 nm/ml assay solution | Oreochromis mossambicus |
additional information | - |
670 units/mg protein | Oreochromis mossambicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | - |
Oreochromis mossambicus | 5'-phosphooligonucleotides + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE, gel filtration | Oreochromis mossambicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
inactivation after 10 min incubation | Oreochromis mossambicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
in the presence of Mg2+ | Oreochromis mossambicus |