Inhibitors | Comment | Organism | Structure |
---|---|---|---|
RNA | enhanced activity by pretreatment with ribonuclease, variety of RNA's including tRNA | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | absolute requirement for Mn2+ or Mg2+ | Escherichia coli | |
Mg2+ | absolute requirement for Mg2+ or Mn2+ | Escherichia coli | |
Mn2+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | Escherichia coli | cleaves both strands of a double helix at or near the same level, about 400 nucleotides removed at each endonucleolytic scission, causes a "shattering" at the point of cleavage, leading to the libration of small oligonucleotides by an exonucleolytic mode of attack | 5'-phosphooligonucleotides + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | degrades native DNA 7fold faster than denatured DNA | Escherichia coli | 5'-phosphooligonucleotides + ? | products have an avarage chain length of 7 residues | ? | |
double-stranded DNA + H2O | cleaves both strands of a double helix at or near the same level, about 400 nucleotides removed at each endonucleolytic scission, causes a "shattering" at the point of cleavage, leading to the libration of small oligonucleotides by an exonucleolytic mode of attack | Escherichia coli | 5'-phosphooligonucleotides + ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Escherichia coli |