Literature summary for 3.1.2.6 extracted from

Stamp, A.L.; Owen, P.; El Omari, K.; Nichols, C.E.; Lockyer, M.; Lamb, H.K.; Charles, I.G.; Hawkins, A.R.; Stammers, D.K.
Structural and functional characterization of Salmonella enterica serovar Typhimurium YcbL: an unusual Type II glyoxalase (2010), Protein Sci., 19, 1897-1905.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)	Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	stimulates activity (0.1 mM: 9-fold)	Salmonella enterica subsp. enterica serovar Typhimurium
Mn2+	stimulates activity (0.1 mM: 5-fold, 1mM: 7-fold)	Salmonella enterica subsp. enterica serovar Typhimurium
Zn2+	bound, molar ratio 0.65 Zn2+:1	Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ion exchange chromatography, ammonium sulfate precipitation, gel filtration, hydroxyapatite chromatography (not retained on the column)	Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-D-lactoylglutathione + H2O	-	Salmonella enterica subsp. enterica serovar Typhimurium	D-lactate + glutathione	-	?

Synonyms

Synonyms	Comment	Organism
glyoxylase II	-	Salmonella enterica subsp. enterica serovar Typhimurium

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Release 2023.1 (January 2023)