Cloned (Comment) | Organism |
---|---|
His-tagged version of mutant protein expressed in Escherichia coli BL21-Codon plus | Leishmania infantum |
Crystallization (Comment) | Organism |
---|---|
- |
Leishmania infantum |
Protein Variants | Comment | Organism |
---|---|---|
Y291R/C294K | substrate specificity changed | Leishmania infantum |
General Stability | Organism |
---|---|
protein stability increased in the presence of 10 mM MnCl2 and 5% PEG (PEG4000, PEG400, PEG8000) | Leishmania infantum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.092 | - |
S-D-lactoyltrypanothione | wild type protein, pH not specified in the publication, temperature not specified in the publication | Leishmania infantum | |
0.15 | - |
S-D-lactoyltrypanothione | Y291R/C294K mutant protein, pH not specified in the publication, temperature not specified in the publication | Leishmania infantum | |
0.439 | - |
S-D-lactoylglutathione | Y291R/C294K mutant protein, pH not specified in the publication, temperature not specified in the publication | Leishmania infantum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | metalloprotein | Leishmania infantum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania infantum | Q2PYN0 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Co2+), gel filtration | Leishmania infantum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-D-lactoylglutathione + H2O | used by mutant Y291R/C294K | Leishmania infantum | D-lactate + glutathione | - |
? | |
S-D-lactoyltrypanothione + H2O | additionally S-D-lactoylglutathione used by mutant Y291R/C294K | Leishmania infantum | D-lactate + trypanothione | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 295 amino acid residues | Leishmania infantum |
Synonyms | Comment | Organism |
---|---|---|
glyoxylase II | - |
Leishmania infantum |
hydroxyacylglutathione hydrolase | - |
Leishmania infantum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16 | - |
S-D-lactoyltrypanothione | wild type protein, no activity observed with S-D-lactoylglutathione, pH not specified in the publication, temperature not specified in the publication | Leishmania infantum | |
241 | - |
S-D-lactoyltrypanothione | Y291R/C294K mutant protein, pH not specified in the publication, temperature not specified in the publication | Leishmania infantum | |
918 | - |
S-D-lactoylglutathione | Y291R/C294K mutant protein, pH not specified in the publication, temperature not specified in the publication | Leishmania infantum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
180 | - |
S-D-lactoyltrypanothione | wild type protein, pH not specified in the publication, temperature not specified in the publication | Leishmania infantum | |
1600 | - |
S-D-lactoyltrypanothione | Y291R/C294K mutant protein, pH not specified in the publication, temperature not specified in the publication | Leishmania infantum | |
2090 | - |
S-D-lactoylglutathione | Y291R/C294K mutant protein, pH not specified in the publication, temperature not specified in the publication | Leishmania infantum |