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Literature summary for 3.1.2.6 extracted from

  • Barata, L.; Silva, M.S.; Schuldt, L.; Ferreira, A.E.; Gomes, R.A.; Tomas, A.M.; Weiss, M.S.; Freire, A.P.; Cordeiro, C.
    Enlightening the molecular basis of trypanothione specificity in trypanosomatids: Mutagenesis of Leishmania infantum glyoxalase II (2011), Exp. Parasitol., 129, 402-408.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged version of mutant protein expressed in Escherichia coli BL21-Codon plus Leishmania infantum

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Leishmania infantum

Protein Variants

Protein Variants Comment Organism
Y291R/C294K substrate specificity changed Leishmania infantum

General Stability

General Stability Organism
protein stability increased in the presence of 10 mM MnCl2 and 5% PEG (PEG4000, PEG400, PEG8000) Leishmania infantum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.092
-
S-D-lactoyltrypanothione wild type protein, pH not specified in the publication, temperature not specified in the publication Leishmania infantum
0.15
-
S-D-lactoyltrypanothione Y291R/C294K mutant protein, pH not specified in the publication, temperature not specified in the publication Leishmania infantum
0.439
-
S-D-lactoylglutathione Y291R/C294K mutant protein, pH not specified in the publication, temperature not specified in the publication Leishmania infantum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metalloprotein Leishmania infantum

Organism

Organism UniProt Comment Textmining
Leishmania infantum Q2PYN0
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Co2+), gel filtration Leishmania infantum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-D-lactoylglutathione + H2O used by mutant Y291R/C294K Leishmania infantum D-lactate + glutathione
-
?
S-D-lactoyltrypanothione + H2O additionally S-D-lactoylglutathione used by mutant Y291R/C294K Leishmania infantum D-lactate + trypanothione
-
?

Subunits

Subunits Comment Organism
monomer 295 amino acid residues Leishmania infantum

Synonyms

Synonyms Comment Organism
glyoxylase II
-
Leishmania infantum
hydroxyacylglutathione hydrolase
-
Leishmania infantum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16
-
S-D-lactoyltrypanothione wild type protein, no activity observed with S-D-lactoylglutathione, pH not specified in the publication, temperature not specified in the publication Leishmania infantum
241
-
S-D-lactoyltrypanothione Y291R/C294K mutant protein, pH not specified in the publication, temperature not specified in the publication Leishmania infantum
918
-
S-D-lactoylglutathione Y291R/C294K mutant protein, pH not specified in the publication, temperature not specified in the publication Leishmania infantum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
180
-
S-D-lactoyltrypanothione wild type protein, pH not specified in the publication, temperature not specified in the publication Leishmania infantum
1600
-
S-D-lactoyltrypanothione Y291R/C294K mutant protein, pH not specified in the publication, temperature not specified in the publication Leishmania infantum
2090
-
S-D-lactoylglutathione Y291R/C294K mutant protein, pH not specified in the publication, temperature not specified in the publication Leishmania infantum