Cloned (Comment) | Organism |
---|---|
cloning of RNA interference constructs for targeting GLX II in bloodstream and procyclic Trypanosoma brucei, cloning of the constructs for the deletion of the GLX II alleles in procyclic cells and cloning of the pHD1701/GLXIImyc2 plasmid for the overexpression of GLX II | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.109 | - |
S-propionyltrypanothione | - |
Trypanosoma brucei | |
0.133 | - |
S-hydroxyglutaryltrypanothione | - |
Trypanosoma brucei | |
0.144 | - |
S-acetyltrypanothione | - |
Trypanosoma brucei | |
0.164 | - |
S-bis-lactoyltrypanothione | - |
Trypanosoma brucei | |
0.195 | - |
S-lactoylglutathionylspermidine | - |
Trypanosoma brucei | |
0.265 | - |
S-glycolyltrypanothione | - |
Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Trypanosoma brucei | recombinant glyoxalase II hydrolyzes the trypanothione-thioesters of methylglyoxal, glyoxal and 4,5-dioxovalerate, substrates of the classical glyoxalase system, with high efficiency | ? | - |
? | |
additional information | Trypanosoma brucei | The glyoxalase system, composed of glyoxalase I and II catalyzes the detoxification of reactive ketoaldehydes and thus protects cells from ketoaldehyde-mediated formation of advanced glycation end products. The main physiological substrate is the toxic and mutagenic methylglyoxal, which originates from triosephosphates during glycolysis as well as threonine catabolism. | ? | - |
? | |
S-acetyltrypanothione + H2O | Trypanosoma brucei | - |
acetate + trypanothione | - |
? | |
S-bis-lactoyltrypanothione + 2 H2O | Trypanosoma brucei | - |
2 D-lactate + trypanothione | - |
? | |
S-glycolyltrypanothione + H2O | Trypanosoma brucei | - |
glycolate + trypanothione | - |
? | |
S-hydroxyglutaryltrypanothione + H2O | Trypanosoma brucei | - |
hydroxyglutarate + trypanothione + 2 H+ | - |
? | |
S-lactoylglutathionylspermidine + H2O | Trypanosoma brucei | - |
? | - |
? | |
S-propionyltrypanothione + H2O | Trypanosoma brucei | - |
propionate + trypanothione + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | Q581U6 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
449 cell | culture-adapted bloodstream and procyclic Trypanosoma brucei of cell line 449, descendants of the strain Lister 427, stably transfected with pHD449 encoding the tetracycline repressor | Trypanosoma brucei | - |
cell culture | for RNA interference experiments a cell line (427 1313 514) is employed that encodes a second tetracycline repressor and constitutively expresses a T7-polymerase | Trypanosoma brucei | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
after the onset of interfering RNAi, GLX II activity in extracts of bloodstream cells is decreased to about 20% when compared to the respective non-induced cells | Trypanosoma brucei |
additional information | - |
overexpression of GLX II has no apparent effect on Trypanosoma brucei | Trypanosoma brucei |
0.04 | - |
clone B2 (pHD678/GLXII) plus tetracycline, GLX II activity in bloodstream Trypanosoma brucei subject to RNA interference | Trypanosoma brucei |
0.05 | - |
clone B4 (p2T7/GLXII) plus tetracycline, GLX II activity in bloodstream Trypanosoma brucei subject to RNA interference | Trypanosoma brucei |
0.21 | - |
wild-type minus tetracycine, GLX II activity in bloodstream Trypanosoma brucei subject to RNA interference | Trypanosoma brucei |
0.22 | - |
clone B4 (p2T7/GLXII) minus tetracycline, GLX II activity in bloodstream Trypanosoma brucei subject to RNA interference | Trypanosoma brucei |
0.22 | - |
clone K3 (pHD678) minus tetracycline, GLX II activity in bloodstream Trypanosoma brucei subject to RNA interference | Trypanosoma brucei |
0.23 | - |
clone K3 (pHD678) plus tetracycline, GLX II activity in bloodstream Trypanosoma brucei subject to RNA interference | Trypanosoma brucei |
0.27 | - |
clone B2 (pHD678/GLXII) minus tetracycline, GLX II activity in bloodstream Trypanosoma brucei subject to RNA interference | Trypanosoma brucei |
63 | - |
S-hydroxyglutaryltrypanothione as substrate, kinetic parameters of recombinant enzyme are determined in 100 mM MOPS | Trypanosoma brucei |
73 | - |
S-glycolyltrypanothione as substrate, kinetic parameters of recombinant enzyme are determined in 100 mM MOPS | Trypanosoma brucei |
92 | - |
S-bis-lactoyltrypanothione as substrate, kinetic parameters of recombinant enzyme are determined in 100 mM MOPS | Trypanosoma brucei |
96 | - |
S-propionyltrypanothione as substrate, kinetic parameters of recombinant enzyme are determined in 100 mM MOPS | Trypanosoma brucei |
115 | - |
S-lactoylglutathionylspermidine as substrate, kinetic parameters of recombinant enzyme are determined in 100 mM MOPS | Trypanosoma brucei |
117 | - |
S-acetyltrypanothione as substrate, kinetic parameters of recombinant enzyme are determined in 100 mM MOPS | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | recombinant glyoxalase II hydrolyzes the trypanothione-thioesters of methylglyoxal, glyoxal and 4,5-dioxovalerate, substrates of the classical glyoxalase system, with high efficiency | Trypanosoma brucei | ? | - |
? | |
additional information | The glyoxalase system, composed of glyoxalase I and II catalyzes the detoxification of reactive ketoaldehydes and thus protects cells from ketoaldehyde-mediated formation of advanced glycation end products. The main physiological substrate is the toxic and mutagenic methylglyoxal, which originates from triosephosphates during glycolysis as well as threonine catabolism. | Trypanosoma brucei | ? | - |
? | |
S-acetyltrypanothione + H2O | - |
Trypanosoma brucei | acetate + trypanothione | - |
? | |
S-bis-lactoyltrypanothione + 2 H2O | - |
Trypanosoma brucei | 2 D-lactate + trypanothione | - |
? | |
S-glycolyltrypanothione + H2O | - |
Trypanosoma brucei | glycolate + trypanothione | - |
? | |
S-hydroxyglutaryltrypanothione + H2O | - |
Trypanosoma brucei | hydroxyglutarate + trypanothione + 2 H+ | - |
? | |
S-lactoylglutathionylspermidine + H2O | - |
Trypanosoma brucei | ? | - |
? | |
S-propionyltrypanothione + H2O | - |
Trypanosoma brucei | propionate + trypanothione + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Glx II | - |
Trypanosoma brucei |
glyoxalase II | - |
Trypanosoma brucei |
hydroxyacylglutathione hydrolase | - |
Trypanosoma brucei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Trypanosoma brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Trypanosoma brucei |