Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor diffusion method at 4°C and 18°C, the crystal structure of GloB solved at 1.4 A | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.241 | - |
S-D-lactoylglutathione | enzyme obtained from minimal medium supplemented with Mn2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.295 | - |
S-D-lactoylglutathione | enzyme obtained from minimal medium supplemented with Zn2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.408 | - |
S-D-lactoylglutathione | enzyme otained from LB medium | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.463 | - |
S-D-lactoylglutathione | enzyme obtained from minimal medium supplemented with Fe2+ | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | proteins expressed in minimal medium supplemented with Zn2+, Mn2+ or Fe2+ show similar activities | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mn2+ | proteins expressed in minimal medium supplemented with Zn2+, Mn2+ or Fe2+ show similar activities | Salmonella enterica subsp. enterica serovar Typhimurium | |
Zn2+ | proteins expressed in minimal medium supplemented with Zn2+, Mn2+ or Fe2+ show similar activities | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | Q8ZRM2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-D-lactoylglutathione | - |
Salmonella enterica subsp. enterica serovar Typhimurium | D-lactate + glutathione | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GloB | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
glyoxalase II | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16 | - |
S-D-lactoylglutathione | enzyme obtained from minimal medium supplemented with Fe2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
26.7 | - |
S-D-lactoylglutathione | enzyme obtained from minimal medium supplemented with Zn2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
149.9 | - |
S-D-lactoylglutathione | enzyme obtained from minimal medium supplemented with Fe2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
168.8 | - |
S-D-lactoylglutathione | enzyme otained from LB medium | Salmonella enterica subsp. enterica serovar Typhimurium | |
209.9 | - |
S-D-lactoylglutathione | enzyme obtained from minimal medium supplemented with Zn2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
394.9 | - |
S-D-lactoylglutathione | enzyme obtained from minimal medium supplemented with Mn2+ | Salmonella enterica subsp. enterica serovar Typhimurium |