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Literature summary for 3.1.2.6 extracted from
- The binding of iron and zinc to glyoxalase II occurs exclusively as di-metal centers and is unique within the metallo-beta-lactamase family (2004), J. Biol. Inorg. Chem., 9, 429-438.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli in minimal medium | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.139 | - |
S-Lactoylglutathione | pH 7.2, 25°C, growth on minimal medium without in presence of zinc | Arabidopsis thaliana |  |
| 0.14 | - |
S-D-lactoylglutathione | recombinant cytosolic isozyme, medium with Zn added, pH 7.2, 25°C | Arabidopsis thaliana | |
| 0.209 | - |
S-Lactoylglutathione | pH 7.2, 25°C, growth on minimal medium without added metal ion | Arabidopsis thaliana | |
| 0.21 | - |
S-D-lactoylglutathione | recombinant cytosolic isozyme, minimal medium, pH 7.2, 25°C | Arabidopsis thaliana | |
| 0.6 | - |
S-D-lactoylglutathione | recombinant cytosolic isozyme, medium with Fe added, pH 7.2, 25°C | Arabidopsis thaliana | |
| 0.6 | - |
S-Lactoylglutathione | pH 7.2, 25°C, growth on minimal medium in presence of iron | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Arabidopsis thaliana | 5829 | - |
| cytosol | cytosolic isozyme | Arabidopsis thaliana | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Arabidopsis thaliana | |
| Iron | 0.55 mol per mol of protein in minimal medium, located in the dinuclear site, metalloenzyme, enzyme binds a mixture of zinc, iron, and manganese when produced in cells grown in rich medium | Arabidopsis thaliana | |
| Mn2+ | - |
Arabidopsis thaliana | |
| Mn2+ | 0.05 mol per mol of protein in minimal medium, metalloenzyme, enzyme binds a mixture of zinc, iron, and manganese when produced in cells grown in rich medium | Arabidopsis thaliana | |
| additional information | metal binding structure, and metal content under different media conditions, overview, enzyme shows positive cooperative metal binding | Arabidopsis thaliana | |
| additional information | overexpresssion of enzyme in minmal media containing either Zn, Fe, or Mn results in enzyme binding an average of 1 equivalent of metal ion, and presence of antiferomagnetically and ferromagnetically coupled dinuclear metal centers. Enzyme does not exist as a mononuclear metal ion containing enzyme, and shows positive cooperativity in metal binding | Arabidopsis thaliana | |
| Zn2+ | - |
Arabidopsis thaliana | |
| Zn2+ | 0.5 mol per mol of protein in minimal medium, located in the dinuclear site, metalloenzyme, enzyme binds a mixture of zinc, iron, and manganese when produced in cells grown in rich medium | Arabidopsis thaliana | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
cytoslic isozyme | - |
| Arabidopsis thaliana | - |
overexpresssion in minmal media containing either Zn, Fe, or Mn | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-D-lactoylglutathione + H2O | - |
Arabidopsis thaliana | glutathione + D-lactate | - |
? | |
| S-lactoylglutathione + H2O | - |
Arabidopsis thaliana | D-lactate + glutathione | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetoacetylglutathione hydrolase | - |
Arabidopsis thaliana |
| Germ cell specific protein | - |
Arabidopsis thaliana |
| GLO II | - |
Arabidopsis thaliana |
| GLX2-2 | - |
Arabidopsis thaliana |
| glyoxalase 2 | - |
Arabidopsis thaliana |
| glyoxalase II | - |
Arabidopsis thaliana |
| hydrolase, acetoacetylglutathione | - |
Arabidopsis thaliana |
| hydrolase, hydroxyacylglutathione | - |
Arabidopsis thaliana |
| More | the enzyme belongs to the metallo-beta-lactamase superfamily | Arabidopsis thaliana |
| Round spermatid protein RSP29 | - |
Arabidopsis thaliana |
| S-2-hydroxylacylglutathione hydrolase | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 136 | - |
S-D-lactoylglutathione | recombinant cytosolic isozyme, medium with Fe added, pH 7.2, 25°C | Arabidopsis thaliana | |
| 136 | - |
S-Lactoylglutathione | pH 7.2, 25°C, growth on minimal medium in presence of iron | Arabidopsis thaliana | |
| 240 | - |
S-D-lactoylglutathione | recombinant cytosolic isozyme, medium with Zn added, pH 7.2, 25°C | Arabidopsis thaliana | |
| 240 | - |
S-Lactoylglutathione | pH 7.2, 25°C, growth on minimal medium without in presence of zinc | Arabidopsis thaliana | |
| 470 | - |
S-D-lactoylglutathione | recombinant cytosolic isozyme, minimal medium, pH 7.2, 25°C | Arabidopsis thaliana | |
| 470 | - |
S-Lactoylglutathione | pH 7.2, 25°C, growth on minimal medium without added metal ion | Arabidopsis thaliana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Arabidopsis thaliana |
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