Any feedback?
Literature summary for 3.1.2.6 extracted from
- Glyoxalase II of African trypanosomes is trypanothione-dependent (2004), J. Biol. Chem., 279, 22209-22217.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no dependance on ionic strength | Trypanosoma brucei |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Trypanosoma brucei |
| DNA and amino acid sequence determination and analysis, overexpression as N-terminally His-tagged and as free enzyme in Escherichia coli | Trypanosoma brucei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is not sensitive against ionic strength | Trypanosoma brucei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.086 | - |
bis-(lactoyl)trypanothione | pH 7.2, 25°C | Trypanosoma brucei |  |
| 0.086 | - |
bis-(lactoyl)trypanothione | recombinant enzyme, pH 7.2, 25°C | Trypanosoma brucei | |
| 0.108 | - |
mono-(lactoyl)trypanothione | pH 7.2, 25°C | Trypanosoma brucei | |
| 0.108 | - |
mono-(lactoyl)trypanothione | recombinant enzyme, pH 7.2, 25°C | Trypanosoma brucei | |
| 3 | - |
S-D-lactoylglutathione | above, recombinant enzyme, pH 7.2, 25°C | Trypanosoma brucei | |
| 3 | - |
lactoylglutathione | or above, pH 7.2, 25°C | Trypanosoma brucei | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cobalt | 0.6 mol per mol of protein | Trypanosoma brucei | |
| Iron | 0.42 mol per mol of protein | Trypanosoma brucei | |
| additional information | the enzyme contains the highly conserved metal binding motif THXHXDH, total metal ions per mol of enzyme: 1.5 for the His-tagged enzyme, 0.7-0.9 for the free enzyme | Trypanosoma brucei | |
| additional information | sequence contains the highly conserved metal binding motif THXHXDH | Trypanosoma brucei | |
| Zinc | 0.45 mol per mol of protein | Trypanosoma brucei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
gel filtration | Trypanosoma brucei |
| 25000 | - |
recombinant enzyme, gel filtration | Trypanosoma brucei |
| 32000 | - |
1 * 32000, recombinant enzyme, SDS-PAGE | Trypanosoma brucei |
| 32000 | - |
1 * 32507, calculated, 1 * 32000, SDS-PAGE | Trypanosoma brucei |
| 32507 | - |
1 * 32507, calculated, 1 * 32000, SDS-PAGE | Trypanosoma brucei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-D-lactoylglutathione + H2O | Trypanosoma brucei | enzyme is involved in the detoxification of ketoaldehydes such as methylglyoxal, which is mainly a byproduct of glycolysis | glutathione + D-lactate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei | Q6KF36 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme with and without His-tag | Trypanosoma brucei |
| recombinant His-tagged and non-tagged enzyme expressed in Escherichia coli | Trypanosoma brucei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Trypanosoma brucei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bis-(lactoyl)trypanothione + H2O | preferred substrate | Trypanosoma brucei | trypanothione + D-lactate | trypathione is bis(glutathionyl)spermidine | ? | |
| bis-(lactoyl)trypanothione + H2O | - |
Trypanosoma brucei | D-lactate + trypanothione | - |
? | |
| lactoylglutathione + H2O | enzyme does not show saturation kinetics up to 5 mM | Trypanosoma brucei | D-lactate + glutathione | - |
? | |
| mono-(lactoyl)trypanothione + H2O | preferred substrate | Trypanosoma brucei | trypanothione + D-lactate | trypathione is bis(glutathionyl)spermidine | ? | |
| mono-(lactoyl)trypanothione + H2O | - |
Trypanosoma brucei | D-lactate + trypanothione | - |
? | |
| S-D-lactoylglutathione + H2O | - |
Trypanosoma brucei | glutathione + D-lactate | - |
? | |
| S-D-lactoylglutathione + H2O | enzyme is involved in the detoxification of ketoaldehydes such as methylglyoxal, which is mainly a byproduct of glycolysis | Trypanosoma brucei | glutathione + D-lactate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 32000, recombinant enzyme, SDS-PAGE | Trypanosoma brucei |
| monomer | 1 * 32507, calculated, 1 * 32000, SDS-PAGE | Trypanosoma brucei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetoacetylglutathione hydrolase | - |
Trypanosoma brucei |
| Germ cell specific protein | - |
Trypanosoma brucei |
| GLO II | - |
Trypanosoma brucei |
| glyoxalase II | - |
Trypanosoma brucei |
| hydrolase, acetoacetylglutathione | - |
Trypanosoma brucei |
| hydrolase, hydroxyacylglutathione | - |
Trypanosoma brucei |
| Round spermatid protein RSP29 | - |
Trypanosoma brucei |
| S-2-hydroxylacylglutathione hydrolase | - |
Trypanosoma brucei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Trypanosoma brucei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | broad | Trypanosoma brucei |
| 7 | 8 | broad optimum | Trypanosoma brucei |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Trypanosoma brucei | calculated from protein sequence | - |
6 |
| Trypanosoma brucei | calculated | 6.5 | 6 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
