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Literature summary for 3.1.2.6 extracted from

  • Schilling, O.; Wenzel, N.; Naylor, M.; Vogel, A.; Crowder, M.; Makaroff, C.; Meyer-Klaucke, W.
    Flexible metal binding of the metallo-beta-lactamase domain: glyoxalase II incorporates iron, manganese, and zinc in vivo (2003), Biochemistry, 42, 11777-11786.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
R248W mutant shows enhanced zinc content and reduced iron content compared to the wild-type enzyme Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ addition to the medium slightly reduces the activity in vivo Arabidopsis thaliana
Mn2+ addition to the medium reduces the activity in vivo Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
S-D-lactoylglutathione recombinant enzyme grown in medium supplemented with Mn2+, pH 7.2, 25°C Arabidopsis thaliana
0.22
-
S-D-lactoylglutathione recombinant enzyme grown in medium not supplemented with metal ions or supplemented with Fe2+, pH 7.2, 25°C Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 0.8 mol per mol of protein, wild-type enzyme, 0.4 mol per mol of protein, R248W mutant enzyme Arabidopsis thaliana
Mn2+ 0.1 mol per mol of protein, R248W mutant enzyme Arabidopsis thaliana
additional information enzyme possesses a binuclear active site, metal ion binding structure and kinetics, overview Arabidopsis thaliana
Zn2+ 0.4 mol per mol of protein, wild-type enzyme, 2.1 mol per mol of protein, R248W mutant enzyme Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O24496 recombinant enzyme expressed in Escherichia coli
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-D-lactoylglutathione + H2O
-
Arabidopsis thaliana glutathione + D-lactate
-
?

Synonyms

Synonyms Comment Organism
acetoacetylglutathione hydrolase
-
Arabidopsis thaliana
Germ cell specific protein
-
Arabidopsis thaliana
GLO II
-
Arabidopsis thaliana
GlxII
-
Arabidopsis thaliana
glyoxalase II
-
Arabidopsis thaliana
hydrolase, acetoacetylglutathione
-
Arabidopsis thaliana
hydrolase, hydroxyacylglutathione
-
Arabidopsis thaliana
More enzyme belongs to the metallo-beta-lactamase superfamily Arabidopsis thaliana
Round spermatid protein RSP29
-
Arabidopsis thaliana
S-2-hydroxylacylglutathione hydrolase
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
168
-
S-D-lactoylglutathione recombinant enzyme grown in medium supplemented with Mn2+, pH 7.2, 25°C Arabidopsis thaliana
359
-
S-D-lactoylglutathione recombinant enzyme grown in medium supplemented with Fe2+, pH 7.2, 25°C Arabidopsis thaliana
408
-
S-D-lactoylglutathione recombinant enzyme grown in medium not supplemented with metal ions, pH 7.2, 25°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Arabidopsis thaliana