Protein Variants | Comment | Organism |
---|---|---|
R248W | mutant shows enhanced zinc content and reduced iron content compared to the wild-type enzyme | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | addition to the medium slightly reduces the activity in vivo | Arabidopsis thaliana | |
Mn2+ | addition to the medium reduces the activity in vivo | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
S-D-lactoylglutathione | recombinant enzyme grown in medium supplemented with Mn2+, pH 7.2, 25°C | Arabidopsis thaliana | |
0.22 | - |
S-D-lactoylglutathione | recombinant enzyme grown in medium not supplemented with metal ions or supplemented with Fe2+, pH 7.2, 25°C | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 0.8 mol per mol of protein, wild-type enzyme, 0.4 mol per mol of protein, R248W mutant enzyme | Arabidopsis thaliana | |
Mn2+ | 0.1 mol per mol of protein, R248W mutant enzyme | Arabidopsis thaliana | |
additional information | enzyme possesses a binuclear active site, metal ion binding structure and kinetics, overview | Arabidopsis thaliana | |
Zn2+ | 0.4 mol per mol of protein, wild-type enzyme, 2.1 mol per mol of protein, R248W mutant enzyme | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O24496 | recombinant enzyme expressed in Escherichia coli | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-D-lactoylglutathione + H2O | - |
Arabidopsis thaliana | glutathione + D-lactate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acetoacetylglutathione hydrolase | - |
Arabidopsis thaliana |
Germ cell specific protein | - |
Arabidopsis thaliana |
GLO II | - |
Arabidopsis thaliana |
GlxII | - |
Arabidopsis thaliana |
glyoxalase II | - |
Arabidopsis thaliana |
hydrolase, acetoacetylglutathione | - |
Arabidopsis thaliana |
hydrolase, hydroxyacylglutathione | - |
Arabidopsis thaliana |
More | enzyme belongs to the metallo-beta-lactamase superfamily | Arabidopsis thaliana |
Round spermatid protein RSP29 | - |
Arabidopsis thaliana |
S-2-hydroxylacylglutathione hydrolase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
168 | - |
S-D-lactoylglutathione | recombinant enzyme grown in medium supplemented with Mn2+, pH 7.2, 25°C | Arabidopsis thaliana | |
359 | - |
S-D-lactoylglutathione | recombinant enzyme grown in medium supplemented with Fe2+, pH 7.2, 25°C | Arabidopsis thaliana | |
408 | - |
S-D-lactoylglutathione | recombinant enzyme grown in medium not supplemented with metal ions, pH 7.2, 25°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Arabidopsis thaliana |