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Literature summary for 3.1.2.6 extracted from

  • Murata, K.; Inoue, Y.; Watanabe, K.; Fukuda, Y.; Saikusa, T.; Shimosaka, M.; Kimura, A.
    Metabolism of alpha-ketoaldehydes in yeast: purification and characterization of glyoxalase II from Saccharomyces cerevisiae (1986), Agric. Biol. Chem., 50, 135-142.
No PubMed abstract available
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Inhibitors

Inhibitors Comment Organism Structure
coenzyme A
-
 Saccharomyces cerevisiae
DTT 1.25 mM, 60% inhibition Saccharomyces cerevisiae
glutathione 8.0 mM, 43% loss of activity Saccharomyces cerevisiae
Hg2+ HgCl2, 0.5 mM, complete inhibition Saccharomyces cerevisiae
iodoacetate
-
 Saccharomyces cerevisiae
PCMB 0.3 mM, complete inhibition Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007
-
 S-Lactoylglutathione
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.34
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactoylglutathione + H2O
-
 Saccharomyces cerevisiae D-lactate + glutathione
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 19000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 pH 7.0, 14 min, 50% loss of activity Saccharomyces cerevisiae
50
-
 pH 7.0, 2 min, 50% loss of activity Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
 stable in 0.001 N HCl at 40°C for 15 min Saccharomyces cerevisiae
9
-
 rapid loss of activity above Saccharomyces cerevisiae