Literature summary for 3.1.2.20 extracted from

Nie, L.; Ren, Y.; Schulz, H.
Identification and characterization of Escherichia coli thioesterase III that functions in fatty acid beta-oxidation (2008), Biochemistry, 47, 7744-7751.

Search for more literature for 3.1.2.20

Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged version expressed in Escherichia coli BL21(DE3)	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.003	-	3,5-cis-tetradecadienoyl-CoA	-	Escherichia coli
0.0058	-	3-hydroxytetradecanoyl-CoA	-	Escherichia coli
0.006	-	palmitoyl-CoA	-	Escherichia coli
0.0063	-	myristoyl-CoA	-	Escherichia coli
0.0108	-	3,5-cis-dodecadienoyl-CoA	-	Escherichia coli
0.013	-	lauroyl-CoA	-	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
15000	-	4 * 15000, crystal structure analysis, SDS-PAGE	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P77712	-	-

Purification (Commentary)

Purification (Comment)	Organism
ion exchange chromatography (DEAE-cellulose), hydroxylapatite chromatography, hydrophobic interaction chromatography, recombinant protein: immobilized metal ion affinity chromatography (Ni2+), ion exchange chromatography (DEAE-cellulose), hydrophobic interaction chromatography	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	also comparable activities observed with stearoyl-CoA and oleoyl-CoA	Escherichia coli
6.9	-	with lauroyl-CoA	Escherichia coli
26.4	-	with myristoyl-CoA	Escherichia coli
43.5	-	with palmitoyl-CoA	Escherichia coli
45.5	-	with 3-hydroxytetradecanoyl-CoA	Escherichia coli
92.3	-	with 3,5-cis-dodecadienoyl-CoA	Escherichia coli
101.3	-	with 3,5-cis-tetradecadienoyl-CoA	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3,5-cis-dodecadienoyl-CoA + H2O	-	Escherichia coli	CoA + 3,5-cis-dodecadienoic acid	-	?
3,5-cis-tetradecadienoyl-CoA + H2O	-	Escherichia coli	CoA + 3,5-cis-tetradecadienoic acid	-	?
3-hydroxytetradecanoyl-CoA + H2O	-	Escherichia coli	CoA + 3-hydroxytetradecanoate	-	?
lauroyl-CoA + H2O	-	Escherichia coli	CoA + laurate	-	?
myristoyl-CoA + H2O	-	Escherichia coli	CoA + myristate	-	?
palmitoyl-CoA + H2O	-	Escherichia coli	CoA + palmitate	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 15000, crystal structure analysis, SDS-PAGE	Escherichia coli

Synonyms

Synonyms	Comment	Organism
thioesterase III	-	Escherichia coli
ybaW protein	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	inactive with substrates having acyl chains with fewer than 12 carbon atoms	Escherichia coli
1.6	-	lauroyl-CoA	-	Escherichia coli
2	3.7	3,5-cis-tetradecadienoyl-CoA	-	Escherichia coli
6.2	-	myristoyl-CoA	-	Escherichia coli
10.2	-	palmitoyl-CoA	-	Escherichia coli
10.7	-	3-hydroxytetradecanoyl-CoA	-	Escherichia coli
21.6	-	3,5-cis-dodecadienoyl-CoA	-	Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
100	-	lauroyl-CoA	-	Escherichia coli
1000	-	myristoyl-CoA	-	Escherichia coli
1700	-	palmitoyl-CoA	-	Escherichia coli
1800	-	3-hydroxytetradecanoyl-CoA	-	Escherichia coli
2000	-	3,5-cis-dodecadienoyl-CoA	-	Escherichia coli
7900	-	3,5-cis-tetradecadienoyl-CoA	-	Escherichia coli

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Release 2023.1 (January 2023)