Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Haemophilus influenzae |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Haemophilus influenzae |
Protein Variants | Comment | Organism |
---|---|---|
D44A | putative catalytic residue | Haemophilus influenzae |
N29A | involved in substrate binding | Haemophilus influenzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0042 | - |
lauroyl-CoA | N29A mutant protein | Haemophilus influenzae | |
0.0066 | - |
lauroyl-CoA | wild-type protein | Haemophilus influenzae | |
0.013 | - |
isobutyryl-CoA | wild-type protein | Haemophilus influenzae | |
0.092 | - |
isobutyryl-CoA | N29A mutant protein | Haemophilus influenzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17000 | - |
6 * 17000, crystal structure analysis | Haemophilus influenzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | P44886 | stain Rd KW20 | - |
Purification (Comment) | Organism |
---|---|
- |
Haemophilus influenzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butyryl-CoA + H2O | - |
Haemophilus influenzae | butanoate + CoA | - |
? | |
butyrylpanthetheine phosphate + H2O | - |
Haemophilus influenzae | pantetheine phosphate + butyrate | - |
? | |
isobutyryl-CoA + H2O | - |
Haemophilus influenzae | CoA + isobutyrate | - |
? | |
lauroyl-CoA + H2O | - |
Haemophilus influenzae | CoA + lauroate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 17000, crystal structure analysis | Haemophilus influenzae |
Synonyms | Comment | Organism |
---|---|---|
acyl-Coenzyme A thioesterase | - |
Haemophilus influenzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | D44A mutant protein: value below 0.001 s-1 for isobutyryl-CoA and lauroyl-CoA | Haemophilus influenzae | |
0.08 | - |
butyrylpantetheine phosphate | wild-type protein | Haemophilus influenzae | |
2.34 | - |
isobutyryl-CoA | N29A mutant protein | Haemophilus influenzae | |
2.57 | - |
lauroyl-CoA | N29A mutant protein | Haemophilus influenzae | |
6.7 | - |
butyryl-CoA | wild-type protein | Haemophilus influenzae | |
9.2 | - |
lauroyl-CoA | wild-type protein | Haemophilus influenzae | |
138 | - |
isobutyryl-CoA | wild-type protein | Haemophilus influenzae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
butyrylpantetheine phosphate | wild-type protein | Haemophilus influenzae | |
25 | - |
isobutyryl-CoA | N29A mutant protein | Haemophilus influenzae | |
100 | - |
butyryl-CoA | wild-type protein | Haemophilus influenzae | |
610 | - |
lauroyl-CoA | N29A mutant protein | Haemophilus influenzae | |
1400 | - |
lauroyl-CoA | wild-type protein | Haemophilus influenzae | |
11000 | - |
isobutyryl-CoA | wild-type protein | Haemophilus influenzae |