Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | movement of a helical lid controls access of substrates to the active site of RifR. The active site of RifR is a classic catalytic triad comprising residues Ser94, Asp200, and His228 | Amycolatopsis mediterranei |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 StarTM | Amycolatopsis mediterranei |
Crystallization (Comment) | Organism |
---|---|
by hanging drop vapor diffusion at 4°C. Two RifR polypeptides in the asymmetric unit (P2(1): a=39.5 A, b=94.6 A, c=63.2 A, beta=90.55°) | Amycolatopsis mediterranei |
Protein Variants | Comment | Organism |
---|---|---|
S94A | thioesterase activity is effectively eliminated for all substrates except with isobutyryl-CoA and propionyl-CoA. No breakdown of [14C]acyl-S639A RifM1 substrates | Amycolatopsis mediterranei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amycolatopsis mediterranei | Q7BUF9 | - |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration and metal affinity column | Amycolatopsis mediterranei |
Storage Stability | Organism |
---|---|
-80°C, 50 mM HEPES buffer, pH 7.5, 50 mM NaCl, 1 mM EDTA, 1 mM tris-(2-carboxyethyl)phosphine, 10% (v/v) glycerol | Amycolatopsis mediterranei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O | - |
Amycolatopsis mediterranei | CoA + acetate | - |
? | |
acetyl-RifM1 + H2O | - |
Amycolatopsis mediterranei | ? | - |
? | |
butyryl-CoA + H2O | - |
Amycolatopsis mediterranei | butanoate + CoA | - |
? | |
decanoyl-CoA + H2O | - |
Amycolatopsis mediterranei | CoA + decanoate | - |
? | |
hexanoyl-CoA + H2O | - |
Amycolatopsis mediterranei | CoA + hexanoate | - |
? | |
isobutyryl-CoA + H2O | - |
Amycolatopsis mediterranei | CoA + isobutyrate | - |
? | |
malonyl-CoA + H2O | - |
Amycolatopsis mediterranei | CoA + malonate | - |
? | |
methylmalonyl-CoA + H2O | - |
Amycolatopsis mediterranei | CoA + methylmalonate | - |
? | |
methylmalonyl-RifM1 + H2O | - |
Amycolatopsis mediterranei | ? | - |
? | |
octanoyl-CoA + H2O | - |
Amycolatopsis mediterranei | CoA + octanoate | - |
? | |
propionyl-CoA + H2O | - |
Amycolatopsis mediterranei | CoA + propionate | - |
? | |
propionyl-RifM1 + H2O | - |
Amycolatopsis mediterranei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | in solution, gel filtration | Amycolatopsis mediterranei |
Synonyms | Comment | Organism |
---|---|---|
RifR | - |
Amycolatopsis mediterranei |
TEII | - |
Amycolatopsis mediterranei |
type II thioesterase | - |
Amycolatopsis mediterranei |