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Literature summary for 3.1.2.20 extracted from

  • Claxton, H.B.; Akey, D.L.; Silver, M.K.; Admiraal, S.J.; Smith, J.L.
    Structure and functional analysis of RifR, the type II thioesterase from the rifamycin biosynthetic pathway (2009), J. Biol. Chem., 284, 5021-5029.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information movement of a helical lid controls access of substrates to the active site of RifR. The active site of RifR is a classic catalytic triad comprising residues Ser94, Asp200, and His228 Amycolatopsis mediterranei

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 StarTM Amycolatopsis mediterranei

Crystallization (Commentary)

Crystallization (Comment) Organism
by hanging drop vapor diffusion at 4°C. Two RifR polypeptides in the asymmetric unit (P2(1): a=39.5 A, b=94.6 A, c=63.2 A, beta=90.55°) Amycolatopsis mediterranei

Protein Variants

Protein Variants Comment Organism
S94A thioesterase activity is effectively eliminated for all substrates except with isobutyryl-CoA and propionyl-CoA. No breakdown of [14C]acyl-S639A RifM1 substrates Amycolatopsis mediterranei

Organism

Organism UniProt Comment Textmining
Amycolatopsis mediterranei Q7BUF9
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration and metal affinity column Amycolatopsis mediterranei

Storage Stability

Storage Stability Organism
-80°C, 50 mM HEPES buffer, pH 7.5, 50 mM NaCl, 1 mM EDTA, 1 mM tris-(2-carboxyethyl)phosphine, 10% (v/v) glycerol Amycolatopsis mediterranei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O
-
Amycolatopsis mediterranei CoA + acetate
-
?
acetyl-RifM1 + H2O
-
Amycolatopsis mediterranei ?
-
?
butyryl-CoA + H2O
-
Amycolatopsis mediterranei butanoate + CoA
-
?
decanoyl-CoA + H2O
-
Amycolatopsis mediterranei CoA + decanoate
-
?
hexanoyl-CoA + H2O
-
Amycolatopsis mediterranei CoA + hexanoate
-
?
isobutyryl-CoA + H2O
-
Amycolatopsis mediterranei CoA + isobutyrate
-
?
malonyl-CoA + H2O
-
Amycolatopsis mediterranei CoA + malonate
-
?
methylmalonyl-CoA + H2O
-
Amycolatopsis mediterranei CoA + methylmalonate
-
?
methylmalonyl-RifM1 + H2O
-
Amycolatopsis mediterranei ?
-
?
octanoyl-CoA + H2O
-
Amycolatopsis mediterranei CoA + octanoate
-
?
propionyl-CoA + H2O
-
Amycolatopsis mediterranei CoA + propionate
-
?
propionyl-RifM1 + H2O
-
Amycolatopsis mediterranei ?
-
?

Subunits

Subunits Comment Organism
monomer in solution, gel filtration Amycolatopsis mediterranei

Synonyms

Synonyms Comment Organism
RifR
-
Amycolatopsis mediterranei
TEII
-
Amycolatopsis mediterranei
type II thioesterase
-
Amycolatopsis mediterranei