Activating Compound | Comment | Organism | Structure |
---|---|---|---|
isopropyl-thio-beta-D-galactoside | inducer | Streptomyces coelicolor |
Cloned (Comment) | Organism |
---|---|
wild-type ScoT and the H224Y mutant expressed in Escherichia coli BL21(DE3)/pLysS cells. Mutants S90A and S90C expressed in Escherichia coli strain BL21Star(DE3). Mutant H210Y expressed in Escherichia coli BL21Star(DE3) cells additionally transformed with plasmid pGroESL encoding chaperones | Streptomyces coelicolor |
Protein Variants | Comment | Organism |
---|---|---|
H210Y | activity is close to that of the wild-type | Streptomyces coelicolor |
H224Y | activity is much lower than that of the wild-type | Streptomyces coelicolor |
S90A | completely abolishes the hydrolytic activity of ScoT | Streptomyces coelicolor |
S90C | exhibits some residual activity | Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
34 | - |
N-acetylcysteamine acetate | at pH 7.5, 30°C | Streptomyces coelicolor | |
52 | - |
N-acetylcysteamine propionate | at pH 7.5, 30°C | Streptomyces coelicolor | |
56 | - |
N-acetylcysteamine butyrate | at pH 7.5, 30°C | Streptomyces coelicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by nickel affinity chromatography followed by anion-exchange chromatography | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | ScoT associated with polyketide synthase Cpk from Streptomyces coelicolor A3 is able to hydrolyze acetyl, propionyl, and butyryl residues. ScoT is an alpha/beta hydrolase with Ser90 and His224 in its active site and it clearly prefers propionate | Streptomyces coelicolor | ? | - |
? | |
N-acetylcysteamine acetate | - |
Streptomyces coelicolor | N-acetylcysteamine + acetate | - |
? | |
N-acetylcysteamine butyrate | - |
Streptomyces coelicolor | N-acetylcysteamine + butyrate | - |
? | |
N-acetylcysteamine propionate | - |
Streptomyces coelicolor | N-acetylcysteamine + propionate | - |
? | |
p-nitrophenyl acetate + H2O | - |
Streptomyces coelicolor | p-nitrophenol + acetate | - |
? | |
p-nitrophenyl butyrate | - |
Streptomyces coelicolor | p-nitrophenol + butyrate | - |
? | |
p-nitrophenyl propionate | - |
Streptomyces coelicolor | p-nitrophenol + propionate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SCOT | - |
Streptomyces coelicolor |
TE II | - |
Streptomyces coelicolor |
type II thioesterase | - |
Streptomyces coelicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
N-acetylcysteamine propionate | at pH 7.5, 30°C | Streptomyces coelicolor | |
1.817 | - |
N-acetylcysteamine butyrate | at pH 7.5, 30°C | Streptomyces coelicolor | |
7.5 | - |
N-acetylcysteamine acetate | at pH 7.5, 30°C | Streptomyces coelicolor |