Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-hydroxybutyrate | stimulates the mitochondrial-matrix enzyme activity | Rattus norvegicus | |
ADP | stimulates the microsomal activity | Rattus norvegicus | |
ATP | stimulates the microsomal activity | Rattus norvegicus | |
citrate | - |
Rattus norvegicus | |
cyanide | - |
Rattus norvegicus | |
cysteine | - |
Rattus norvegicus | |
dithiothreitol | stimulation of the microsomal enzyme | Rattus norvegicus | |
EDTA | stimulates the activity of the mitochondrial enzyme above 2 mM | Rattus norvegicus | |
FAD | stimulates the activity of the mitochondrial-matrix enzyme up to 0.4 mM | Rattus norvegicus | |
FMN | stimulates the microsomal activity | Rattus norvegicus | |
fumarate | stimulates the microsomal activity | Rattus norvegicus | |
malate | stimulates the mitochondrial-matrix enzyme activity | Rattus norvegicus | |
NAD+ | stimulates the microsomal activity | Rattus norvegicus | |
nicotinic acid | stimulates the microsomal activity | Rattus norvegicus | |
riboflavin | - |
Rattus norvegicus | |
succinate | stimulates the mitochondrial-matrix enzyme activity | Rattus norvegicus |
General Stability | Organism |
---|---|
thawing and freezing inactivates | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | inhibits the mitochondrial-matrix enzyme | Rattus norvegicus | |
ATP | inhibits the mitochondrial-matrix enzyme | Rattus norvegicus | |
beta-hydroxybutyrate | moderately inhibits the microsomal enzyme activity | Rattus norvegicus | |
Ca2+ | inhibits the microsomal enzyme | Rattus norvegicus | |
Co2+ | slightly inhibits the mitochondrial enzyme | Rattus norvegicus | |
Cu2+ | - |
Rattus norvegicus | |
EDTA | inhibits the activity of the mitochondrial enzyme at concentrations of 0.5 mM | Rattus norvegicus | |
F- | - |
Rattus norvegicus | |
FAD | slightly inhibits the microsomal enzyme | Rattus norvegicus | |
FMN | inhibits the mitochondrial-matrix enzyme activity | Rattus norvegicus | |
fumarate | inhibits the mitochondrial-matrix enzyme activity | Rattus norvegicus | |
KCl | inhibits the mitochondrial enzyme above 125 mM | Rattus norvegicus | |
Mg2+ | inhibits the microsomal enzyme, at higher concentrations also the mitochondrial enzyme | Rattus norvegicus | |
Mn2+ | - |
Rattus norvegicus | |
NAD+ | inhibits the mitochondrial-matrix enzyme | Rattus norvegicus | |
nicotinic acid | inhibits the mitochondrial-matrix enzyme activity | Rattus norvegicus | |
NO3- | - |
Rattus norvegicus | |
Pb2+ | slightly inhibits the mitochondrial enzyme | Rattus norvegicus | |
SO42- | - |
Rattus norvegicus | |
Triton X-100 | - |
Rattus norvegicus | |
Zn2+ | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
hexadecanoyl-CoA | - |
Rattus norvegicus | |
0.047 | - |
hexadecanoyl-CoA | for the microsomal enzyme | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Rattus norvegicus | |
Mg2+ | stimulates the mitochondrial enzyme by 0.5mM | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + H2O | Rattus norvegicus | - |
CoA + palmitate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.063 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O | - |
Rattus norvegicus | CoA + acetate | - |
? | |
acyl-CoA + H2O | long-chain fatty-acyl-CoA | Rattus norvegicus | CoA + a carboxylate | - |
? | |
cholesteryl oleate + H2O | - |
Rattus norvegicus | cholesterol + oleate | - |
? | |
dipalmitoylglycero-3-phosphocholine + H2O | - |
Rattus norvegicus | ? | - |
? | |
dodecanoyl-CoA + H2O | i.e. lauroyl-CoA | Rattus norvegicus | CoA + dodecanoate | - |
? | |
hexadecanoyl-CoA + H2O | best substrate | Rattus norvegicus | CoA + hexadecanoate | - |
? | |
hexadecanoyl-CoA + H2O | i.e. palmitoyl-CoA | Rattus norvegicus | CoA + hexadecanoate | - |
? | |
octadecanoyl-CoA + H2O | i.e. stearoyl-CoA | Rattus norvegicus | CoA + octadecanoate | - |
? | |
oleoyl-CoA + H2O | - |
Rattus norvegicus | CoA + oleate | - |
? | |
palmitoyl-CoA + H2O | - |
Rattus norvegicus | CoA + palmitate | - |
? | |
tetradecanoyl-CoA + H2O | i.e. myristoyl-CoA | Rattus norvegicus | CoA + tetradecanoate | - |
? | |
tripalmitoylglycerol + H2O | - |
Rattus norvegicus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
stable | Rattus norvegicus |
45 | - |
microsomal enzyme: stable, mitochondrial enzyme: unstable | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.2 | - |
Rattus norvegicus |