Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.2.2 extracted from

  • Berge, R.K.; Dossland, B.
    Differences between microsomal and mitochondrial-matrix palmitoyl-coenzyme A hydrolase, and palmitoyl-L-carnitine hydrolase from rat liver (1979), Biochem. J., 181, 119-125.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
3-hydroxybutyrate stimulates the mitochondrial-matrix enzyme activity Rattus norvegicus
ADP stimulates the microsomal activity Rattus norvegicus
ATP stimulates the microsomal activity Rattus norvegicus
citrate
-
Rattus norvegicus
cyanide
-
Rattus norvegicus
cysteine
-
Rattus norvegicus
dithiothreitol stimulation of the microsomal enzyme Rattus norvegicus
EDTA stimulates the activity of the mitochondrial enzyme above 2 mM Rattus norvegicus
FAD stimulates the activity of the mitochondrial-matrix enzyme up to 0.4 mM Rattus norvegicus
FMN stimulates the microsomal activity Rattus norvegicus
fumarate stimulates the microsomal activity Rattus norvegicus
malate stimulates the mitochondrial-matrix enzyme activity Rattus norvegicus
NAD+ stimulates the microsomal activity Rattus norvegicus
nicotinic acid stimulates the microsomal activity Rattus norvegicus
riboflavin
-
Rattus norvegicus
succinate stimulates the mitochondrial-matrix enzyme activity Rattus norvegicus

General Stability

General Stability Organism
thawing and freezing inactivates Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
ADP inhibits the mitochondrial-matrix enzyme Rattus norvegicus
ATP inhibits the mitochondrial-matrix enzyme Rattus norvegicus
beta-hydroxybutyrate moderately inhibits the microsomal enzyme activity Rattus norvegicus
Ca2+ inhibits the microsomal enzyme Rattus norvegicus
Co2+ slightly inhibits the mitochondrial enzyme Rattus norvegicus
Cu2+
-
Rattus norvegicus
EDTA inhibits the activity of the mitochondrial enzyme at concentrations of 0.5 mM Rattus norvegicus
F-
-
Rattus norvegicus
FAD slightly inhibits the microsomal enzyme Rattus norvegicus
FMN inhibits the mitochondrial-matrix enzyme activity Rattus norvegicus
fumarate inhibits the mitochondrial-matrix enzyme activity Rattus norvegicus
KCl inhibits the mitochondrial enzyme above 125 mM Rattus norvegicus
Mg2+ inhibits the microsomal enzyme, at higher concentrations also the mitochondrial enzyme Rattus norvegicus
Mn2+
-
Rattus norvegicus
NAD+ inhibits the mitochondrial-matrix enzyme Rattus norvegicus
nicotinic acid inhibits the mitochondrial-matrix enzyme activity Rattus norvegicus
NO3-
-
Rattus norvegicus
Pb2+ slightly inhibits the mitochondrial enzyme Rattus norvegicus
SO42-
-
Rattus norvegicus
Triton X-100
-
Rattus norvegicus
Zn2+
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
hexadecanoyl-CoA
-
Rattus norvegicus
0.047
-
hexadecanoyl-CoA for the microsomal enzyme Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Rattus norvegicus
-
-
mitochondrion
-
Rattus norvegicus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
Rattus norvegicus
Mg2+ stimulates the mitochondrial enzyme by 0.5mM Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
palmitoyl-CoA + H2O Rattus norvegicus
-
CoA + palmitate
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.063
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O
-
Rattus norvegicus CoA + acetate
-
?
acyl-CoA + H2O long-chain fatty-acyl-CoA Rattus norvegicus CoA + a carboxylate
-
?
cholesteryl oleate + H2O
-
Rattus norvegicus cholesterol + oleate
-
?
dipalmitoylglycero-3-phosphocholine + H2O
-
Rattus norvegicus ?
-
?
dodecanoyl-CoA + H2O i.e. lauroyl-CoA Rattus norvegicus CoA + dodecanoate
-
?
hexadecanoyl-CoA + H2O best substrate Rattus norvegicus CoA + hexadecanoate
-
?
hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Rattus norvegicus CoA + hexadecanoate
-
?
octadecanoyl-CoA + H2O i.e. stearoyl-CoA Rattus norvegicus CoA + octadecanoate
-
?
oleoyl-CoA + H2O
-
Rattus norvegicus CoA + oleate
-
?
palmitoyl-CoA + H2O
-
Rattus norvegicus CoA + palmitate
-
?
tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Rattus norvegicus CoA + tetradecanoate
-
?
tripalmitoylglycerol + H2O
-
Rattus norvegicus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
stable Rattus norvegicus
45
-
microsomal enzyme: stable, mitochondrial enzyme: unstable Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.2
-
Rattus norvegicus