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Literature summary for 3.1.2.2 extracted from

  • Tilton, G.B.; Shockey, J.M.; Browse, J.
    Biochemical and molecular characterization of ACH2, an acyl-CoA thioesterase from arabidopsis thaliana (2004), J. Biol. Chem., 279, 7487-7494.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis of isolated and commercial clone, overexpression of His-tagged isozyme ACH2 in Escherichia coli BL21(DE3) cells Arabidopsis thaliana
gene ACH2, DNA and amino acid sequence determination and analysis, overexpression as C-terminally His-tagged protein in Escherichia coli BL21(DE3) Arabidopsis thaliana
overexpression in Escherichia coli BL-21(DE3) as a His-tagged protein Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
acyl-CoA e.g. lauroyl-CoA, at 0.005 mM, can be overcome by addition of bovine serum albumin or alpha-casein Arabidopsis thaliana
CoASH feedback inhibition of recombinant enzyme at concentrations above 0.1 mM Arabidopsis thaliana
DTNB above 0.3 mM; above 0.3 mM Arabidopsis thaliana
additional information reversible substrate inhibition by long-chain acyl-CoAs at concentration 0.005 mM, reversibel by bovine serum albumin and alpha-casein Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Arabidopsis thaliana
0.0058
-
oleoyl-CoA
-
Arabidopsis thaliana
0.0058
-
palmitoleoyl-CoA pH 8.0 Arabidopsis thaliana
0.0178
-
dodecanoyl-CoA
-
Arabidopsis thaliana
0.018
-
dodecanoyl-CoA pH 8.0 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome isozyme ACH2 Arabidopsis thaliana 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
196000
-
recombinant isozyme ACH2, analytical ultracentrifugation Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + H2O Arabidopsis thaliana enzyme is not linked to fatty acid oxidation CoA + a carboxylate
-
?
palmitoleoyl-CoA + H2O Arabidopsis thaliana activity of ACH2 is not linked to fatty acid oxidation CoA + palmitoleate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
isozyme ACH2
-
Arabidopsis thaliana
-
Wassilewskija ecotype
-
Arabidopsis thaliana Q8GYW7
-
-
Arabidopsis thaliana Q8GYW7 isozyme ACH2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Arabidopsis thaliana
recombinant His-tagged isozyme ACH2 from Escherichia coli Arabidopsis thaliana

Reaction

Reaction Comment Organism Reaction ID
palmitoyl-CoA + H2O = CoA + palmitate catalytic triad D337-Q409-S359 Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Arabidopsis thaliana
-
leaf young Arabidopsis thaliana
-
additional information expression level during plant development Arabidopsis thaliana
-
seed
-
Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21
-
purified recombinant enzyme, substrate palmitoleoyl-CoA Arabidopsis thaliana
21
-
activity with palmitoleoyl-CoA Arabidopsis thaliana

Storage Stability

Storage Stability Organism
4°C, pH 6.0, activity of recombinant His-tagged enzyme remains constant for 3 days Arabidopsis thaliana
4°C, pH 6.0, fully stable for 3 days, 90% remaining activity at pH 8.0 after 3 days Arabidopsis thaliana
4°C, pH 8.0, 3 days, recombinant His-tagged enzyme retains 90% of the original activity Arabidopsis thaliana
4°C, purified recombinant HIs-tagged enzyme, pH 6.0, no loss of activity after 3 days Arabidopsis thaliana
4°C, purified recombinant HIs-tagged enzyme, pH 8.0, 90% remaining activity after 3 days Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + H2O prefers unsaturated acyl-CoA Arabidopsis thaliana CoA + a carboxylate
-
?
acyl-CoA + H2O long-chain fatty-acyl-CoA Arabidopsis thaliana CoA + a carboxylate
-
?
acyl-CoA + H2O enzyme is not linked to fatty acid oxidation Arabidopsis thaliana CoA + a carboxylate
-
?
arachidonoyl-CoA + H2O
-
Arabidopsis thaliana CoA + arachidonate
-
?
dodecanoyl-CoA + H2O
-
Arabidopsis thaliana CoA + dodecanoate
-
?
dodecanoyl-CoA + H2O i.e. lauroyl-CoA Arabidopsis thaliana CoA + dodecanoate
-
?
lauroyl-CoA + H2O
-
Arabidopsis thaliana CoA + laurate
-
?
linoleoyl-CoA + H2O
-
Arabidopsis thaliana CoA + linoleoate
-
?
additional information broad substrate specificity, preference for long-chain unsaturated fatty acids Arabidopsis thaliana ?
-
?
myristoyl-CoA + H2O
-
Arabidopsis thaliana CoA + myristate
-
?
oleoyl-CoA + H2O
-
Arabidopsis thaliana CoA + oleate
-
?
palmitoleoyl-CoA
-
Arabidopsis thaliana CoA + CoA + palmitoleoate
-
?
palmitoleoyl-CoA + H2O
-
Arabidopsis thaliana CoA + palmitoleoate
-
?
palmitoleoyl-CoA + H2O best substrate Arabidopsis thaliana CoA + palmitoleoate
-
?
palmitoleoyl-CoA + H2O
-
Arabidopsis thaliana CoA + palmitoleate
-
?
palmitoleoyl-CoA + H2O activity of ACH2 is not linked to fatty acid oxidation Arabidopsis thaliana CoA + palmitoleate
-
?
palmitoyl-CoA + H2O
-
Arabidopsis thaliana CoA + palmitate
-
?
stearoyl-CoA + H2O
-
Arabidopsis thaliana CoA + stearate
-
?

Subunits

Subunits Comment Organism
tetramer
-
Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
ACH2
-
Arabidopsis thaliana
acyl-CoA hydrolase
-
Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.5 pH 7.5: about 40% of maximal activity, pH 9.5: about 40% of maximal activity Arabidopsis thaliana