Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis of isolated and commercial clone, overexpression of His-tagged isozyme ACH2 in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
gene ACH2, DNA and amino acid sequence determination and analysis, overexpression as C-terminally His-tagged protein in Escherichia coli BL21(DE3) | Arabidopsis thaliana |
overexpression in Escherichia coli BL-21(DE3) as a His-tagged protein | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acyl-CoA | e.g. lauroyl-CoA, at 0.005 mM, can be overcome by addition of bovine serum albumin or alpha-casein | Arabidopsis thaliana | |
CoASH | feedback inhibition of recombinant enzyme at concentrations above 0.1 mM | Arabidopsis thaliana | |
DTNB | above 0.3 mM; above 0.3 mM | Arabidopsis thaliana | |
additional information | reversible substrate inhibition by long-chain acyl-CoAs at concentration 0.005 mM, reversibel by bovine serum albumin and alpha-casein | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Arabidopsis thaliana | |
0.0058 | - |
oleoyl-CoA | - |
Arabidopsis thaliana | |
0.0058 | - |
palmitoleoyl-CoA | pH 8.0 | Arabidopsis thaliana | |
0.0178 | - |
dodecanoyl-CoA | - |
Arabidopsis thaliana | |
0.018 | - |
dodecanoyl-CoA | pH 8.0 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | isozyme ACH2 | Arabidopsis thaliana | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
196000 | - |
recombinant isozyme ACH2, analytical ultracentrifugation | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + H2O | Arabidopsis thaliana | enzyme is not linked to fatty acid oxidation | CoA + a carboxylate | - |
? | |
palmitoleoyl-CoA + H2O | Arabidopsis thaliana | activity of ACH2 is not linked to fatty acid oxidation | CoA + palmitoleate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
isozyme ACH2 | - |
Arabidopsis thaliana | - |
Wassilewskija ecotype | - |
Arabidopsis thaliana | Q8GYW7 | - |
- |
Arabidopsis thaliana | Q8GYW7 | isozyme ACH2 | - |
Purification (Comment) | Organism |
---|---|
recombinant | Arabidopsis thaliana |
recombinant His-tagged isozyme ACH2 from Escherichia coli | Arabidopsis thaliana |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
palmitoyl-CoA + H2O = CoA + palmitate | catalytic triad D337-Q409-S359 | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Arabidopsis thaliana | - |
leaf | young | Arabidopsis thaliana | - |
additional information | expression level during plant development | Arabidopsis thaliana | - |
seed | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21 | - |
purified recombinant enzyme, substrate palmitoleoyl-CoA | Arabidopsis thaliana |
21 | - |
activity with palmitoleoyl-CoA | Arabidopsis thaliana |
Storage Stability | Organism |
---|---|
4°C, pH 6.0, activity of recombinant His-tagged enzyme remains constant for 3 days | Arabidopsis thaliana |
4°C, pH 6.0, fully stable for 3 days, 90% remaining activity at pH 8.0 after 3 days | Arabidopsis thaliana |
4°C, pH 8.0, 3 days, recombinant His-tagged enzyme retains 90% of the original activity | Arabidopsis thaliana |
4°C, purified recombinant HIs-tagged enzyme, pH 6.0, no loss of activity after 3 days | Arabidopsis thaliana |
4°C, purified recombinant HIs-tagged enzyme, pH 8.0, 90% remaining activity after 3 days | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + H2O | prefers unsaturated acyl-CoA | Arabidopsis thaliana | CoA + a carboxylate | - |
? | |
acyl-CoA + H2O | long-chain fatty-acyl-CoA | Arabidopsis thaliana | CoA + a carboxylate | - |
? | |
acyl-CoA + H2O | enzyme is not linked to fatty acid oxidation | Arabidopsis thaliana | CoA + a carboxylate | - |
? | |
arachidonoyl-CoA + H2O | - |
Arabidopsis thaliana | CoA + arachidonate | - |
? | |
dodecanoyl-CoA + H2O | - |
Arabidopsis thaliana | CoA + dodecanoate | - |
? | |
dodecanoyl-CoA + H2O | i.e. lauroyl-CoA | Arabidopsis thaliana | CoA + dodecanoate | - |
? | |
lauroyl-CoA + H2O | - |
Arabidopsis thaliana | CoA + laurate | - |
? | |
linoleoyl-CoA + H2O | - |
Arabidopsis thaliana | CoA + linoleoate | - |
? | |
additional information | broad substrate specificity, preference for long-chain unsaturated fatty acids | Arabidopsis thaliana | ? | - |
? | |
myristoyl-CoA + H2O | - |
Arabidopsis thaliana | CoA + myristate | - |
? | |
oleoyl-CoA + H2O | - |
Arabidopsis thaliana | CoA + oleate | - |
? | |
palmitoleoyl-CoA | - |
Arabidopsis thaliana | CoA + CoA + palmitoleoate | - |
? | |
palmitoleoyl-CoA + H2O | - |
Arabidopsis thaliana | CoA + palmitoleoate | - |
? | |
palmitoleoyl-CoA + H2O | best substrate | Arabidopsis thaliana | CoA + palmitoleoate | - |
? | |
palmitoleoyl-CoA + H2O | - |
Arabidopsis thaliana | CoA + palmitoleate | - |
? | |
palmitoleoyl-CoA + H2O | activity of ACH2 is not linked to fatty acid oxidation | Arabidopsis thaliana | CoA + palmitoleate | - |
? | |
palmitoyl-CoA + H2O | - |
Arabidopsis thaliana | CoA + palmitate | - |
? | |
stearoyl-CoA + H2O | - |
Arabidopsis thaliana | CoA + stearate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
ACH2 | - |
Arabidopsis thaliana |
acyl-CoA hydrolase | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | pH 7.5: about 40% of maximal activity, pH 9.5: about 40% of maximal activity | Arabidopsis thaliana |