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Literature summary for 3.1.2.2 extracted from

  • Yabusaki, K.K.; Ballou, C.E.
    Long-chain fatty acyl-CoA thioesterase from Mycobacterium smegmatis (1981), Methods Enzymol., 71, 242-246.
No PubMed abstract available
Search for more literature for 3.1.2.2

General Stability

General Stability Organism
ovalbumin stabilizes Mycolicibacterium smegmatis

Inhibitors

Inhibitors Comment Organism Structure
3-O-methylmannose polysaccharide
-
 Mycolicibacterium smegmatis
6-O-methylglucose polysaccharide
-
 Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00013 0.006 fatty-acyl-CoA thioester
-
 Mycolicibacterium smegmatis
0.005
-
 hexadecanoyl-CoA micellar Mycolicibacterium smegmatis
0.009
-
 hexadecanoyl-CoA
-
 Mycolicibacterium smegmatis
0.077
-
 CoA
-
 Mycolicibacterium smegmatis
0.107
-
 CoA
-
 Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 2 * 20000, gel electrophoresis Mycolicibacterium smegmatis
40000
-
 x * 40000, SDS-PAGE Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
palmitoyl-CoA + H2O Mycolicibacterium smegmatis
-
 CoA + palmitate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
8000fold Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
230
-
-
 Mycolicibacterium smegmatis

Storage Stability

Storage Stability Organism
4°C in the presence of 10-20% glycerol Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dodecanoyl-CoA + H2O i.e. lauroyl-CoA Mycolicibacterium smegmatis CoA + dodecanoate
-
 ?
hexadecanoyl-CoA + H2O best substrate Mycolicibacterium smegmatis CoA + hexadecanoate
-
 ?
hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Mycolicibacterium smegmatis CoA + hexadecanoate
-
 ?
octadecanoyl-CoA + H2O i.e. stearoyl-CoA Mycolicibacterium smegmatis CoA + octadecanoate
-
 ?
palmitoyl-CoA + H2O
-
 Mycolicibacterium smegmatis CoA + palmitate
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Mycolicibacterium smegmatis
dimer 2 * 20000, gel electrophoresis Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Mycolicibacterium smegmatis