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Literature summary for 3.1.2.14 extracted from
- Effect of a mutagenized acyl-ACP thioesterase FATA allele from sunflower with improved activity in tobacco leaves and Arabidopsis seeds (2014), Planta, 239, 669-677.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Tobacco, Arabidopsis thaliana and Escherichia coli | Helianthus annuus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| L118W | mutant displays three orders of magnitude lower catalytic activity compared to wild-type. Km value similar to wild-type | Helianthus annuus |
| M206W | mutant displays lower catalytic activity compared to wild-type. Km value similar to wild-type, only for substrate 18:1-ACP Km value highly increased compared to wild-type | Helianthus annuus |
| Q215W | mutant displays equivalent or improved activities towards all substrates with marked 2 and 2.6fold increased activities for palmitoleoyl-ACP and palmitoyl-ACP, respectively, compared to wild-type. Expression of mutant in Nicotiana benthamiana leads to a higher average values of acyl-CoA. Expressed in Arabidopsis mutant displays displays a 40% decrease in oil content relative to wild-type. Mutant produces an increase in the content of saturated fatty acids, palmitic and stearic acids, with a concomitant decrease in oleic acid derivatives | Helianthus annuus |
| T182W | mutant displays lower catalytic activity compared to wild-type. Km value similar to wild-type. Leaf triacylglycerols are increased 2 to 4fold when expressed in Nicotiana benthamiana compared to expression of mutant T182W and wild-type, respectively | Helianthus annuus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C, mutant Q215W | Helianthus annuus |  |
| 0.0002 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C, mutant M206W | Helianthus annuus | |
| 0.0003 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C, mutant L118W | Helianthus annuus | |
| 0.0003 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C, wild-type | Helianthus annuus | |
| 0.0004 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C, mutant M206W | Helianthus annuus | |
| 0.0006 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C, mutant L118W | Helianthus annuus | |
| 0.0006 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C, mutant T182W | Helianthus annuus | |
| 0.0006 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C, mutant T182W | Helianthus annuus | |
| 0.0007 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C, mutant L118W | Helianthus annuus | |
| 0.0007 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C, wild-type | Helianthus annuus | |
| 0.0009 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C, mutant Q215W | Helianthus annuus | |
| 0.0009 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C, mutant T182W | Helianthus annuus | |
| 0.0009 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C, wild-type | Helianthus annuus | |
| 0.001 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C, mutant L118W | Helianthus annuus | |
| 0.001 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C, wild-type | Helianthus annuus | |
| 0.0012 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C, mutant M206W | Helianthus annuus | |
| 0.0015 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C, mutant T182W | Helianthus annuus | |
| 0.0022 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C, mutant Q215W | Helianthus annuus | |
| 0.0025 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C, mutant Q215W | Helianthus annuus | |
| 0.0212 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C, mutant M206W | Helianthus annuus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
SDS-PAGE | Helianthus annuus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helianthus annuus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using metal affinity chromatography | Helianthus annuus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oleoyl-[acyl-carrier-protein] + H2O | - |
Helianthus annuus | oleic acid + acyl-carrier-protein | - |
? | |
| palmitoleoyl-[acyl-carrier protein] + H2O | - |
Helianthus annuus | palmitoleate + acyl-carrier protein | - |
? | |
| palmitoyl-[acyl-carrier protein] + H2O | - |
Helianthus annuus | palmitic acid + acyl-carrier protein | - |
? | |
| stearoyl-[acyl-carrier protein] + H2O | - |
Helianthus annuus | stearic acid + acyl-carrier protein | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HaFatA | - |
Helianthus annuus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Helianthus annuus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C, mutant M206W | Helianthus annuus | |
| 0.0005 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C, mutant M206W | Helianthus annuus | |
| 0.0016 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C, mutant M206W | Helianthus annuus | |
| 0.002 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C, mutant T182W | Helianthus annuus | |
| 0.0024 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C, mutant Q215W | Helianthus annuus | |
| 0.003 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C, mutant T182W | Helianthus annuus | |
| 0.0041 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C, mutant Q215W | Helianthus annuus | |
| 0.0062 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C, mutant Q215W | Helianthus annuus | |
| 0.016 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C, mutant T182W | Helianthus annuus | |
| 0.032 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C, mutant T182W | Helianthus annuus | |
| 0.0854 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C, mutant Q215W | Helianthus annuus | |
| 0.1258 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C, mutant M206W | Helianthus annuus | |
| 0.2 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C, mutant L118W | Helianthus annuus | |
| 0.2 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C, mutant L118W | Helianthus annuus | |
| 0.6 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C, mutant L118W | Helianthus annuus | |
| 0.8 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C, wild-type | Helianthus annuus | |
| 1.1 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C, wild-type | Helianthus annuus | |
| 2.2 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C, mutant L118W | Helianthus annuus | |
| 8 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C, wild-type | Helianthus annuus | |
| 35.6 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C, wild-type | Helianthus annuus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Helianthus annuus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | null, wild type and high-efficiency alleles are transiently expressed in tobacco leaves to check their effect on lipid biosynthesis. Expression of active FatA thioesterases alters the composition of leaf triacylglycerols but does not alter total lipid content. The expression of the wild type and the high-efficiency alleles in Arabidopsis thaliana transgenic seeds results in a strong reduction in oil content and an increase in total saturated fatty acid content | Helianthus annuus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C, mutant T182W | Helianthus annuus | |
| 3 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C, mutant T182W | Helianthus annuus | |
| 28 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C, mutant T182W | Helianthus annuus | |
| 50 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C, mutant T182W | Helianthus annuus | |
| 200 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C, mutant L118W | Helianthus annuus | |
| 300 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C, mutant L118W | Helianthus annuus | |
| 400 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C, mutant M206W | Helianthus annuus | |
| 500 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C, mutant M206W | Helianthus annuus | |
| 1100 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C, wild-type | Helianthus annuus | |
| 1200 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C, wild-type | Helianthus annuus | |
| 1600 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C, mutant Q215W | Helianthus annuus | |
| 1800 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C, mutant L118W | Helianthus annuus | |
| 2800 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C, mutant Q215W | Helianthus annuus | |
| 3700 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C, mutant L118W | Helianthus annuus | |
| 5900 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C, mutant M206W | Helianthus annuus | |
| 6600 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C, mutant M206W | Helianthus annuus | |
| 23400 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C, wild-type | Helianthus annuus | |
| 39000 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C, wild-type | Helianthus annuus | |
| 39300 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C, mutant Q215W | Helianthus annuus | |
| 48000 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C, mutant Q215W | Helianthus annuus | |
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