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Literature summary for 3.1.2.14 extracted from
- Acyl-ACP thioesterases from Camelina sativa: Cloning, enzymatic characterization and implication in seed oil fatty acid composition (2014), Phytochemistry, 107, 7-15.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Camelina sativa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C | Camelina sativa |  |
| 0.0005 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C | Camelina sativa | |
| 0.0015 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C | Camelina sativa | |
| 0.0022 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C | Camelina sativa | |
| 0.0031 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C | Camelina sativa | |
| 0.0039 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C | Camelina sativa | |
| 0.0039 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C | Camelina sativa | |
| 0.0066 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C | Camelina sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33400 | - |
calculated from cDNA | Camelina sativa |
| 34800 | - |
calculated from cDNA | Camelina sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Camelina sativa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using affinity chromatography | Camelina sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Camelina sativa | - |
| hypocotyl | - |
Camelina sativa | - |
| leaf | - |
Camelina sativa | - |
| root | - |
Camelina sativa | - |
| seed | - |
Camelina sativa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oleoyl-[acyl-carrier-protein] + H2O | - |
Camelina sativa | oleic acid + acyl-carrier-protein | - |
? | |
| palmitoleoyl-[acyl-carrier protein] + H2O | - |
Camelina sativa | palmitoleate + acyl-carrier protein | - |
? | |
| palmitoyl-[acyl-carrier protein] + H2O | - |
Camelina sativa | palmitic acid + acyl-carrier protein | - |
? | |
| stearoyl-[acyl-carrier protein] + H2O | - |
Camelina sativa | stearic acid + acyl-carrier protein | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FatA | - |
Camelina sativa |
| FatB | - |
Camelina sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Camelina sativa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C | Camelina sativa | |
| 0.1 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C | Camelina sativa | |
| 0.3 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C | Camelina sativa | |
| 0.4 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C | Camelina sativa | |
| 0.6 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C | Camelina sativa | |
| 1 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C | Camelina sativa | |
| 4.4 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C | Camelina sativa | |
| 7 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C | Camelina sativa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Camelina sativa |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Camelina sativa | highly expressed in developing seed endosperm | up |
| Camelina sativa | highly expressed in developing seed endosperm and leaf | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the main change in the Escherichia coli fatty acid composition when CsFatA is expressed is the reduction in unsaturated fatty acids, mainly cis-vaccenic acid (18:1 omega7). This reduction is compensated for by an increase of palmitoleic acid (16:1 omega7) | Camelina sativa |
| metabolism | the main change in the Escherichia coli fatty acid composition when CsFatB is expressed is a decrease in saturated fatty acids and in particular, that of palmitic acid (16:0) that is compensated for with an increase in stearic acid (18.0) | Camelina sativa |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 30 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C | Camelina sativa | |
| 200 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C | Camelina sativa | |
| 200 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C | Camelina sativa | |
| 300 | - |
palmitoleoyl-[acyl-carrier protein] | pH 8, 20°C | Camelina sativa | |
| 300 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C | Camelina sativa | |
| 600 | - |
palmitoyl-[acyl-carrier protein] | pH 8, 20°C | Camelina sativa | |
| 750 | - |
stearoyl-[acyl-carrier protein] | pH 8, 20°C | Camelina sativa | |
| 1800 | - |
oleoyl-[acyl-carrier-protein] | pH 8, 20°C | Camelina sativa | |
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