KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
oleoyl-[acyl-carrier protein] | pH 9.0, 35°C | Brassica rapa subsp. oleifera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Brassica rapa subsp. oleifera |
72000 | - |
gel filtration | Brassica rapa subsp. oleifera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica rapa subsp. oleifera | - |
cv. B-54, Agrani | - |
Purification (Comment) | Organism |
---|---|
native enzyme to homogeneity from developing seeds by hydroxyapatite, ion exchange, and [acyl carrier protein] affinity chromatographies, and preparative native PAGE | Brassica rapa subsp. oleifera |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | developing, fatty acid composition, overview | Brassica rapa subsp. oleifera | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is long-chain fatty acid specific | Brassica rapa subsp. oleifera | ? | - |
? | |
oleoyl-[acyl-carrier protein] + H2O | preferred substrate | Brassica rapa subsp. oleifera | [acyl-carrier protein] + oleate | - |
? | |
stearoyl-[acyl-carrier protein] + H2O | low activity | Brassica rapa subsp. oleifera | [acyl-carrier protein] + stearate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, SDS-PAGE | Brassica rapa subsp. oleifera |
Synonyms | Comment | Organism |
---|---|---|
ACP-thioesterase | - |
Brassica rapa subsp. oleifera |
acyl-acyl carrier protein-thioesterase | - |
Brassica rapa subsp. oleifera |
LC-FAT | - |
Brassica rapa subsp. oleifera |
oleoyl-ACP thioesterase | - |
Brassica rapa subsp. oleifera |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Brassica rapa subsp. oleifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Brassica rapa subsp. oleifera |