Cloned (Comment) | Organism |
---|---|
into the pET-15b vector, cloned into Escherichia coli DH5alpha, subsequently transformed into Escherichia coli strain BL21(DE3) | Salmonella enterica subsp. enterica serovar Typhimurium |
into the pET-15b vector, transformed into Escherichia coli Novablue, subsequently transformed into Escherichia coli strain BL21(DE3) | Streptococcus pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Streptococcus pneumoniae | C6GKN7 | strains T4 and TIGR4 | - |
Purification (Comment) | Organism |
---|---|
by histidine affinity chromatography and an AKTA HPLC system | Salmonella enterica subsp. enterica serovar Typhimurium |
by histidine affinity chromatography and an AKTA HPLC system | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(A) + H2O | 35-nucleotide poly(A) chain, RNase II final end product contains four nucleotides, while RNase R renders a two-nucleotide fragment as the final product | Salmonella enterica subsp. enterica serovar Typhimurium | 5'-AMP + oligonucleotide | - |
? | |
poly(A) + H2O | 35-nucleotide poly(A) chain, RNase R renders a two-nucleotide fragment as the final product | Streptococcus pneumoniae | 5'-AMP + oligonucleotide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase II | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
RNase R | - |
Streptococcus pneumoniae |
RNaseR | - |
Salmonella enterica subsp. enterica serovar Typhimurium |