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Literature summary for 3.1.13.1 extracted from

  • Domingues, S.; Matos, R.G.; Reis, F.P.; Fialho, A.M.; Barbas, A.; Arraiano, C.M.
    Biochemical characterization of the RNase II family of exoribonucleases from the human pathogens Salmonella typhimurium and Streptococcus pneumoniae (2009), Biochemistry, 48, 11848-11857.
    View publication on PubMed
Search for more literature for 3.1.13.1

Cloned(Commentary)

Cloned (Comment) Organism
into the pET-15b vector, cloned into Escherichia coli DH5alpha, subsequently transformed into Escherichia coli strain BL21(DE3) Salmonella enterica subsp. enterica serovar Typhimurium
into the pET-15b vector, transformed into Escherichia coli Novablue, subsequently transformed into Escherichia coli strain BL21(DE3) Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Streptococcus pneumoniae C6GKN7 strains T4 and TIGR4
-

Purification (Commentary)

Purification (Comment) Organism
by histidine affinity chromatography and an AKTA HPLC system Salmonella enterica subsp. enterica serovar Typhimurium
by histidine affinity chromatography and an AKTA HPLC system Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(A) + H2O 35-nucleotide poly(A) chain, RNase II final end product contains four nucleotides, while RNase R renders a two-nucleotide fragment as the final product Salmonella enterica subsp. enterica serovar Typhimurium 5'-AMP + oligonucleotide
-
 ?
poly(A) + H2O 35-nucleotide poly(A) chain, RNase R renders a two-nucleotide fragment as the final product Streptococcus pneumoniae 5'-AMP + oligonucleotide
-
 ?

Synonyms

Synonyms Comment Organism
RNase II
-
 Salmonella enterica subsp. enterica serovar Typhimurium
RNase R
-
 Streptococcus pneumoniae
RNaseR
-
 Salmonella enterica subsp. enterica serovar Typhimurium