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Literature summary for 3.1.13.1 extracted from

  • Kumagai, H.; Igarshi, K.; Tanaka, K.; Nakao, H.; Hirose, S.
    A microsomal exoribonuclease from rat liver (1979), Biochim. Biophys. Acta, 566, 192-199.
    View publication on PubMed
Search for more literature for 3.1.13.1

Activating Compound

Activating Compound Comment Organism Structure
spermine activates in absence of K+ the poly(C)- and poly(A)-breakdown Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
monovalent or divalent cations high concentrations; Mg2+-concentrations above 2 mM Rattus norvegicus
p-chloromercuribenzoate
-
 Rattus norvegicus
Poly(G)
-
 Rattus norvegicus
spermine slight inhibition of poly(U)-degradation Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ optimum for poly(C) as substrate: 25 mM Rattus norvegicus
K+ optimum for poly(A) as substrate: 100 mM Rattus norvegicus
K+ necessary for activity Rattus norvegicus
Mg2+ necessary for activity Rattus norvegicus
Mg2+ optimum: 1-2 mM Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 SDS-PAGE Rattus norvegicus
83000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ss RNA + H2O Rattus norvegicus
-
 5'-phosphomononucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
100fold Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates acts in a processive manner Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information poly(G) is not hydrolyzed Rattus norvegicus ?
-
 ?
poly(A) + H2O
-
 Rattus norvegicus 5'-AMP + oligonucleotide
-
 ?
poly(C) + H2O
-
 Rattus norvegicus 5'-CMP + oligonucleotide
-
 ?
poly(U) + H2O
-
 Rattus norvegicus 5'-UMP + oligonucleotide
-
 ?
ss RNA + H2O
-
 Rattus norvegicus 5'-phosphomononucleotides
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 for 1 min, no activity remains Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Rattus norvegicus