Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-capped mRNA | - |
Saccharomyces cerevisiae | |
Rna with a terminal 5'-phosphate group | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
ribosome | - |
Saccharomyces cerevisiae | 5840 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | necessary for activity | Saccharomyces cerevisiae | |
Mg2+ | optimum: 1.5 mM | Saccharomyces cerevisiae | |
Mn2+ | necessary for activity | Saccharomyces cerevisiae | |
Mn2+ | optimum: 0.3 mM, stimulation about two-thirds the rate with Mg2+ | Saccharomyces cerevisiae | |
NH4+ | activating | Saccharomyces cerevisiae | |
NH4+ | stimulates at pH 9.5 about 100%, optimum: 50 mM | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ss RNA + H2O | Saccharomyces cerevisiae | - |
5'-phosphomononucleotides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
200fold | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates | acts in a processive manner | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | capped mRNA is not hydrolyzed | Saccharomyces cerevisiae | ? | - |
? | |
poly(A) + H2O | - |
Saccharomyces cerevisiae | 5'-AMP + oligonucleotide | - |
? | |
ss RNA + H2O | - |
Saccharomyces cerevisiae | 5'-phosphomononucleotides | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | - |
Saccharomyces cerevisiae |