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Literature summary for 3.1.13.1 extracted from

  • Gupta, R.S.; Kasai, T.; Schlessinger, D.
    Purification and some novel properties of Escherichia coli RNase II (1977), J. Biol. Chem., 252, 8945-8949.
    View publication on PubMed
Search for more literature for 3.1.13.1

Inhibitors

Inhibitors Comment Organism Structure
Na+ substrate: artificial polynucleotides Escherichia coli
Poly(U) strong competitive inhibitor of T4 mRNA-degradation Escherichia coli
rRNA weak inhibitor of T4 mRNA-degradation Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activation against poly(A), -(U), -(C), RNA and T4 mRNA Escherichia coli
Li+ can substitute for K+ to a small extend Escherichia coli
Na+ increases activity against T4 mRNA, can substitute for K+ in activation against T4 mRNA and RNA Escherichia coli
NH4+ activating Escherichia coli
NH4+ can substitute for K+ to a small extend Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000 90000 native and SDS-PAGE, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ss RNA + H2O Escherichia coli
-
 5'-phosphomononucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to apparent homogeneity, 270fold Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.04
-
 activity of the most active fraction, substrate: T4 mRNA Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, small aliquots of purified enzyme, 10% glycerol, stable for at least 19 months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ss RNA + H2O
-
 Escherichia coli 5'-phosphomononucleotides
-
 ?
T4 mRNA + H2O
-
 Escherichia coli 5'-phosphomononucleotides
-
 ?