Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.13.1 extracted from

  • Schmidt, F.J.; McClain, W.H.
    An Escherichia coli ribonuclease which removes an extra nucleotide from a biosynthetic intermediate of bacteriophage T4 proline transfer RNA (1978), Nucleic Acids Res., 5, 4129-4139.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.13.1

Inhibitors

Inhibitors Comment Organism Structure
monovalent or divalent cations 10 mM of Mg2+ reduce activity 2fold, activity barely detectable at 20 mM Mg2+; high concentrations Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Escherichia coli 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ necessary for activity Escherichia coli
Mg2+ optimum: 2 mM Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
precursor tRNA + H2O Escherichia coli
-
 mature tRNA + 5'-phosphomononucleotides
-
 ?
ss RNA + H2O Escherichia coli
-
 5'-phosphomononucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification, between 50-100fold Escherichia coli

Storage Stability

Storage Stability Organism
-70°C, stable for at least 1 month without loss of activity Escherichia coli
0°C, half-life of 5-7 days Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
precursor tRNA + H2O
-
 Escherichia coli mature tRNA + 5'-phosphomononucleotides
-
 ?
ss RNA + H2O
-
 Escherichia coli 5'-phosphomononucleotides
-
 ?
tRNA + H2O RNase BN Escherichia coli ?
-
 ?