Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | acetate has no effect | Escherichia coli | |
phosphate | stimulates | Escherichia coli | |
sulfate | stimulates | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
wild-type and mutant ExoVII (XseA/B homologs TM1768 and TM1769) coexpressed in Escherichia coli BL21(DE3) cells | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
D235A/D240A | is completely inactive, even in the presence of magnesium | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | completely inhibits | Thermotoga maritima | |
additional information | is not active in standard Escherichia coli ExoVII buffer. Acetate has no effect | Thermotoga maritima | |
phosphate | inhibits nuclease activity at 80°C; strongly inhibits, almost complete inhibition by 50 mM | Thermotoga maritima | |
sulfate | strongly inhibits, almost complete inhibition by 50 mM | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | ExoVII activity is maximal at low salt concentrations (lower than 250 mM) | Thermotoga maritima | |
Mg2+ | robust nuclease activity at 80°C is magnesium dependent | Thermotoga maritima | |
additional information | manganese, nickel, cobalt and zinc have no effect | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant ExoVII (XseA/B homologs TM1768 and TM1769) purified by gel filtration and on nickel-NTA column | Thermotoga maritima |
wild-type and mutant ExoVII (XseA/B homologs TM1768 and TM1769) purified on nickel-NTA column | Thermotoga maritima |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
221 | - |
- |
Thermotoga maritima |
267 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | active site motif with two conserved aspartate residues in the large (XseA/TM1768) subunit. Residues Asp235 and Asp240 are essential for the nuclease activity | Thermotoga maritima | ? | - |
? | |
additional information | no significant activity on dsDNA. Active site motif with two conserved aspartate residues in the large (XseA/TM1768) subunit. Residues Asp235 and Asp240 are essential for the nuclease activity | Thermotoga maritima | ? | - |
? | |
single-stranded DNA + H2O | ExoVII activity is highly processive. It binds ssDNA ends and cleaves its substrate endonucleolytically, releasing oligonucleotide products of various sizes | Thermotoga maritima | ? | - |
? | |
single-stranded DNA + H2O | releases oligonucleotide products | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | stoichiometry of small to large subunits regulates ExoVII activity | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
exonuclease VII | - |
Escherichia coli |
exonuclease VII | - |
Thermotoga maritima |
XseA | - |
Thermotoga maritima |
XseB | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
XseA/B homologs TM1768 and TM1769 show robust and maximal nuclease activity | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
XseA/B homologs TM1768 and TM1769 show robust nuclease activity | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
- |
Thermotoga maritima |