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Literature summary for 3.1.11.6 extracted from
- Exonuclease VII of Escherichia coli. Purification and properties (1974), J. Biol. Chem., 249, 4545-4552.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | required for 100% activity | Escherichia coli |  |
| phosphate | 5-10fold higher activity compared to Tris-HCL buffer | Escherichia coli | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | can funtion in absence of divalent cations; slight inhibition | Escherichia coli | |
| N-ethylmaleimide | 47% inhibition in absence of mercaptoethanol | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 25% activation | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88000 | - |
gel fltration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| single-stranded DNA + H2O | Escherichia coli | - |
hydrolyzed single-stranded DNA | oligonucleotides | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
xseA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 1700fold | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| duplex DNA containing single-stranded termini + H2O | - |
Escherichia coli | hydrolyzed single-stranded DNA | - |
? | |
| single-stranded DNA + H2O | - |
Escherichia coli | hydrolyzed single-stranded DNA | oligonucleotides | ? | |
| single-stranded DNA + H2O | no activity on circular DNA | Escherichia coli | hydrolyzed single-stranded DNA | exclusively oligonucleotides | ? | |
| single-stranded DNA + H2O | processive degradation | Escherichia coli | hydrolyzed single-stranded DNA | exclusively oligonucleotides | ? | |
| single-stranded DNA + H2O | no activity on DNA-RNA hybrid molecules | Escherichia coli | hydrolyzed single-stranded DNA | exclusively oligonucleotides | ? | |
| single-stranded DNA + H2O | heat-denatured DNA | Escherichia coli | hydrolyzed single-stranded DNA | exclusively oligonucleotides | ? | |
| single-stranded DNA + H2O | no activity on RNA | Escherichia coli | hydrolyzed single-stranded DNA | exclusively oligonucleotides | ? | |
| thymine dimers + H2O | possible role in repair of ultraviolet damage | Escherichia coli | hydrolyzed single-stranded DNA | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
half-life: 90 min | Escherichia coli |
| 45 | - |
half-life: 45 min | Escherichia coli |
| 60 | - |
half-life: 7 min | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 7.9 | - |
Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 7.8 | 50% loss of activity compared to pH 7.8 | Escherichia coli |
| 7.8 | 8.6 | 50% loss of activity compared to pH 7.8 | Escherichia coli |
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