Cloned (Comment) | Organism |
---|---|
overexpression of RecBCD in Escherichia coli strain SCK387 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli JM109 | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
RecBCD exonuclease | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | type I DNA restriction/modification systems are oligomeric enzymes capable of switching between a methyltransferase function on hemimethylated host DNA and an endonuclease function on unmethylated foreign DNA. Reuse of the methyltransferase subunits is possible so that restriction proceeds until the restriction subunits are depleted. RecBCD exonuclease halts restriction and does not assist recycling, influence of RecBCD on the EcoKI type I restriction enzyme, overview | Escherichia coli |