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Literature summary for 3.1.11.5 extracted from

  • Roberts, G.; Cooper, L.; White, J.; Su, T.; Zipprich, J.; Geary, P.; Kennedy, C.; Dryden, D.
    An investigation of the structural requirements for ATP hydrolysis and DNA cleavage by the EcoKI type I DNA restriction and modification enzyme (2011), Nucleic Acids Res., 39, 7667-7676.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of RecBCD in Escherichia coli strain SCK387 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli JM109
-
-
-

Synonyms

Synonyms Comment Organism
RecBCD exonuclease
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli

General Information

General Information Comment Organism
physiological function type I DNA restriction/modification systems are oligomeric enzymes capable of switching between a methyltransferase function on hemimethylated host DNA and an endonuclease function on unmethylated foreign DNA. Reuse of the methyltransferase subunits is possible so that restriction proceeds until the restriction subunits are depleted. RecBCD exonuclease halts restriction and does not assist recycling, influence of RecBCD on the EcoKI type I restriction enzyme, overview Escherichia coli