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Literature summary for 3.1.11.5 extracted from
- The nuclease domain of the Escherichia coli RecBCD enzyme catalyzes degradation of linear and circular single-stranded and double-stranded DNA (2006), Biochemistry, 45, 131-140.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 134 | - |
34-mer ssDNA | 25°C | Escherichia coli | |
| 315 | - |
Double-stranded DNA | 25°C | Escherichia coli |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight activation of ssDNA nuclease activity of RecB30 | Escherichia coli | |
| Co2+ | activation of ssDNA nuclease activity of RecB30 | Escherichia coli | |
| Cu2+ | slight activation of ssDNA nuclease activity of RecB30 | Escherichia coli | |
| Mg2+ | activation of ssDNA nuclease activity of RecB30 | Escherichia coli | |
| Mn2+ | activation of ssDNA nuclease activity of RecB30 | Escherichia coli | |
| Ni2+ | slight activation of ssDNA nuclease activity of RecB30 | Escherichia coli | |
| Zn2+ | slight activation of ssDNA nuclease activity of RecB30 | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 34-mer ssDNA + H2O | REcB30 binds more tightly on ssDNA than on dsDNA but is more active on dsDNA than on ssDNA | Escherichia coli | ? | - |
? | |
| double-stranded DNA + H2O | - |
Escherichia coli | ? | - |
? | |
| dsDNA + H2O | REcB30 binds more tightly on ssDNA than on dsDNA but is more active on dsDNA than on ssDNA | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| REcB30 protein | universal nuclease domain of REcBCD | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
34-mer ssDNA | 25°C | Escherichia coli | |
| 3.7 | - |
Double-stranded DNA | 25°C | Escherichia coli | |
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