Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3',5'-cyclic AMP | less effective than ATP | Escherichia coli | |
5'-AMP | less effective than ATP | Escherichia coli | |
adenine | less effective than ATP | Escherichia coli | |
adenosine | less effective than ATP | Escherichia coli | |
ADP | less effective than ATP | Escherichia coli | |
CTP | less effective than ATP | Escherichia coli | |
dCTP | less effective than ATP | Escherichia coli | |
GTP | less effective than ATP | Escherichia coli | |
S-adenosylmethionine | less effective than ATP | Escherichia coli | |
TTP | less effective than ATP | Escherichia coli | |
UTP | less effective than ATP | Escherichia coli |
Application | Comment | Organism |
---|---|---|
analysis | possible role in genetic recombination | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
ATP | - |
Escherichia coli | |
0.048 | - |
dATP | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli | |
Mn2+ | less effective than Mg2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
350000 | - |
sedimentation, calculated | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | Escherichia coli | responsible for several steps in genetic recombination process | single-stranded DNA fragments | - |
? | |
double-stranded DNA + H2O | Escherichia coli | ATP-dependent exonuclease | single-stranded DNA fragments | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
mutants lacking the recB or recC or recD gene | - |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.024 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
0°C, Tris, MgCl2, EDTA, mercaptoethanol, 1 d, 5-10% loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Escherichia coli | ADP + phosphate | - |
? | |
double-stranded DNA + H2O | acts on native, heat-denatured and glucosylated DNA | Escherichia coli | single-stranded DNA fragments | - |
? | |
double-stranded DNA + H2O | responsible for several steps in genetic recombination process | Escherichia coli | single-stranded DNA fragments | - |
? | |
double-stranded DNA + H2O | ATP-dependent exonuclease | Escherichia coli | single-stranded DNA fragments | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | - |
Escherichia coli |