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Literature summary for 3.1.1.8 extracted from

  • Saeed, A.; De Boeck, St.; Debruyne, I.; Wouters, J.; Stockx, J.
    Hen's egg yolk cholinesterase (1980), Biochim. Biophys. Acta, 614, 389-399.
    View publication on PubMed
Search for more literature for 3.1.1.8

Activating Compound

Activating Compound Comment Organism Structure
citrate 20 mM, 140% activity Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
N,N,N',N'-tetramethyl-1,6-hexane diamine
-
 Gallus gallus
NaCl
-
 Gallus gallus
physostigmine i.e. eserine Gallus gallus
tetrabutylammonium competitive Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activates Gallus gallus
Mg2+ 20 mM activates Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
104000
-
 yolk and liver cholinesterase 2, gel filtration Gallus gallus
440000
-
 yolk and liver cholinesterase 1, blood plasma cholinesterase, gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Anas platyrhynchos
-
-
-
Anser sp.
-
-
-
Gallus gallus
-
 hen
-
Pavo sp.
-
-
-
pheasant
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, 2 yolk, 2 liver and 1 blood plasma enzyme Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
egg
-
 Anas platyrhynchos
-
egg
-
 Anser sp.
-
egg
-
 Pavo sp.
-
egg
-
 pheasant
-
egg egg yolk Gallus gallus
-
liver laying hens Gallus gallus
-
plasma
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Gallus gallus

Storage Stability

Storage Stability Organism
-16°C, enzyme retains full activity as lyophilized powder after 6 months Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-beta-methylcholine + H2O poor substrate Gallus gallus acetate + beta-methylcholine
-
 ?
acetyl-beta-methylcholine + H2O acetyl-beta-methylcholine chloride Gallus gallus acetate + beta-methylcholine
-
 ?
acetylcholine + H2O acetylcholine chloride Gallus gallus acetate + choline
-
 ?
acetylthiocholine + H2O acetylthiocholine iodide Gallus gallus acetate + thiocholine
-
 ?
benzoylcholine + H2O benzoylcholine chloride Gallus gallus benzoate + choline
-
 ?
butyrylcholine + H2O butyrylcholine chloride Gallus gallus butanoate + choline
-
 ?
butyrylthiocholine + H2O butyrylthiocholine iodide Gallus gallus butanoate + thiocholine
-
 ?
propionylcholine + H2O propionylcholine chloride Gallus gallus propanoate + choline
-
 ?
propionylthiocholine + H2O propionylthiocholine iodide Gallus gallus propanoate + thiocholine
-
 ?
succinyl dicholine dichloride + H2O yolk cholinesterase 1 and 2 and blood plasma cholinesterase poor substrate Gallus gallus ?
-
 ?
succinyl dicholine dichloride + H2O liver cholinesterase 1 and 2 no substrate Gallus gallus ?
-
 ?

Synonyms

Synonyms Comment Organism
acylcholine acyl-hydrolase
-
 Gallus gallus
acylcholine acyl-hydrolase
-
 Anas platyrhynchos
acylcholine acyl-hydrolase
-
 Anser sp.
acylcholine acyl-hydrolase
-
 Pavo sp.
acylcholine acyl-hydrolase
-
 pheasant

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 10 min, pH 7.2, complete loss of activity Gallus gallus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 25°C, excellent stability Gallus gallus