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Literature summary for 3.1.1.8 extracted from

  • Masson, P.; Froment, M.; Gillon, E.; Nachon, F.; Darvesh, S.; Schopfer, L.M.
    Kinetic analysis of butyrylcholinesterase-catalyzed hydrolysis of acetanilides (2007), Biochim. Biophys. Acta, 1774, 1139-1147.
    View publication on PubMed
Search for more literature for 3.1.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in CHO-K1 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D70G site-directed mutagenesis, peripheral site mutant, altered kinetics compared to the wild-type enzyme and no substrate activation, overview Homo sapiens
S198C site-directed mutagenesis, inactive catalytic site mutant Homo sapiens
S198D site-directed mutagenesis, inactive catalytic site mutant Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Echothiophate an organophosphorous compound, inhibition kinetics, overview Homo sapiens
additional information mo product inhibition with aryl acetylamides Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics with aryl acetylamide substrates, overview, low affinity, first order kinetics with neutral amides Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
butyrylthiocholine + H2O Homo sapiens
-
 thiocholine + butyrate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from CHO-K1 cells by anion exchange and affinity chromatography Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
an acylcholine + H2O = choline + a carboxylate the catalytic residue is Ser198 Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2,2-trifluoro-N-(2-nitrophenyl)acetamide + H2O a neutral amide Homo sapiens 2-nitroaniline + trifluoroacetate
-
 ?
2-nitroacetanilide + H2O a neutral amide Homo sapiens 2-nitroaniline + acetate
-
 ?
3-(acetamido) N,N,N-trimethylanilinium + H2O a positively charged amide, hydrolysis by the wild-type enzyme and the mutant D70G shows a long transient phase preceeding the steady state, hysteretic burst induction phase, overview Homo sapiens 3-amino-N,N,N-trimethylanilinium + acetate
-
 ?
3-nitroacetanilide + H2O a neutral amide Homo sapiens 3-nitroaniline + acetate
-
 ?
butyrylthiocholine + H2O
-
 Homo sapiens thiocholine + butyrate
-
 ?
additional information BChE also shows aryl acylamidase activity, i.e. AAA activity, the rate-determining step of which is the acylation, overview Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
monomer recombinant wild-type enzyme Homo sapiens
tetramer native wild-type enzyme Homo sapiens

Synonyms

Synonyms Comment Organism
butyrylcholinesterase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 assay at Homo sapiens