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Literature summary for 3.1.1.76 extracted from
- Characterization of a novel subgroup of extracellular medium-chain-length polyhydroxyalkanoate depolymerases from actinobacteria (2012), Appl. Environ. Microbiol., 78, 7229-7237.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene phaZSomSO2, DNA and amino acid sequence determination and analysis | Streptomyces omiyaensis |
| gene phaZSroSL3, DNA and amino acid sequence determination and analysis | Streptomyces roseolus |
| gene phaZSveSO1, DNA and amino acid sequence determination and analysis | Streptomyces venezuelae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudomonas fluorescens | - |
- |
| extracellular | - |
Pseudomonas alcaligenes | - |
- |
| extracellular | - |
Streptomyces venezuelae | - |
- |
| extracellular | - |
Rhodococcus equi | - |
- |
| extracellular | - |
Stenotrophomonas maltophilia | - |
- |
| extracellular | - |
Streptomyces atratus | - |
- |
| extracellular | - |
Streptomyces anulatus | - |
- |
| extracellular | - |
Streptomyces omiyaensis | - |
- |
| extracellular | - |
Streptomyces beijiangensis | - |
- |
| extracellular | - |
Streptomyces pulveraceus | - |
- |
| extracellular | - |
Streptomyces roseolus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
native PAGE | Streptomyces roseolus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Stenotrophomonas maltophilia | no activity with poly(ethylene succinate), poly(L-lactide), and poly(epsilon-caprolactone), substrate specificity, overview | ? | - |
? |  |
| additional information | Stenotrophomonas maltophilia | no activity with poly(ethylene succinate), poly(L-lactide), poly(3-hydroxybutyrate), and poly(epsilon-caprolactone), substrate specificity, overview | ? | - |
? | |
| additional information | Rhodococcus equi | no activity with poly(ethylene succinate), poly(L-lactide), poly(3-hydroxybutyrate), poly(3-hydroxybutyrate-co-3-hydroxyvalerate), and poly(epsilon-caprolactone), substrate specificity, overview | ? | - |
? | |
| additional information | Pseudomonas alcaligenes | no activity with poly(ethylene succinate), poly(L-lactide), poly(3-hydroxybutyrate-co-3-hydroxyvalerate), and poly(3-hydroxybutyrate), substrate specificity, overview | ? | - |
? | |
| additional information | Pseudomonas fluorescens | no activity with poly(ethylene succinate), poly(L-lactide), poly(3-hydroxypropionate), poly(3-hydroxybutyrate), and poly(3-hydroxybutyrate-co-3-hydroxyvalerate), substrate specificity, overview | ? | - |
? | |
| additional information | Streptomyces atratus | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), EC 3.1.1.75, but not with poly(ethylene succinate), poly(L-lactide), and poly(epsilon-caprolactone), substrate specificity in vivo, overview | ? | - |
? | |
| additional information | Streptomyces anulatus | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), EC 3.1.1.75, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | ? | - |
? | |
| additional information | Streptomyces omiyaensis | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), EC 3.1.1.75, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | ? | - |
? | |
| additional information | Streptomyces roseolus | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), EC 3.1.1.75, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | ? | - |
? | |
| additional information | Streptomyces beijiangensis | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate), EC 3.1.1.75, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | ? | - |
? | |
| additional information | Streptomyces pulveraceus | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate), EC 3.1.1.75, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | ? | - |
? | |
| additional information | Stenotrophomonas maltophilia W1 | no activity with poly(ethylene succinate), poly(L-lactide), and poly(epsilon-caprolactone), substrate specificity, overview | ? | - |
? | |
| additional information | Stenotrophomonas maltophilia W1 | no activity with poly(ethylene succinate), poly(L-lactide), poly(3-hydroxybutyrate), and poly(epsilon-caprolactone), substrate specificity, overview | ? | - |
? | |
| additional information | Pseudomonas alcaligenes SL1 | no activity with poly(ethylene succinate), poly(L-lactide), poly(3-hydroxybutyrate-co-3-hydroxyvalerate), and poly(3-hydroxybutyrate), substrate specificity, overview | ? | - |
? | |
| additional information | Streptomyces anulatus SL11 | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), EC 3.1.1.75, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | ? | - |
? | |
| additional information | Rhodococcus equi W2 | no activity with poly(ethylene succinate), poly(L-lactide), poly(3-hydroxybutyrate), poly(3-hydroxybutyrate-co-3-hydroxyvalerate), and poly(epsilon-caprolactone), substrate specificity, overview | ? | - |
? | |
| additional information | Streptomyces omiyaensis SO2 CECT 7923 | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), EC 3.1.1.75, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | ? | - |
? | |
| additional information | Streptomyces roseolus SL3 CECT 7919 | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), EC 3.1.1.75, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | ? | - |
? | |
| additional information | Streptomyces atratus SL2 | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), EC 3.1.1.75, but not with poly(ethylene succinate), poly(L-lactide), and poly(epsilon-caprolactone), substrate specificity in vivo, overview | ? | - |
? | |
| additional information | Stenotrophomonas maltophilia SL6 | no activity with poly(ethylene succinate), poly(L-lactide), and poly(epsilon-caprolactone), substrate specificity, overview | ? | - |
? | |
| additional information | Stenotrophomonas maltophilia SL6 | no activity with poly(ethylene succinate), poly(L-lactide), poly(3-hydroxybutyrate), and poly(epsilon-caprolactone), substrate specificity, overview | ? | - |
? | |
| additional information | Streptomyces pulveraceus W3 | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate), EC 3.1.1.75, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | ? | - |
? | |
| additional information | Streptomyces beijiangensis SL15 | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate), EC 3.1.1.75, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | ? | - |
? | |
| additional information | Pseudomonas fluorescens GK13 | no activity with poly(ethylene succinate), poly(L-lactide), poly(3-hydroxypropionate), poly(3-hydroxybutyrate), and poly(3-hydroxybutyrate-co-3-hydroxyvalerate), substrate specificity, overview | ? | - |
? | |
| poly(3-hydroxybutyrate) + H2O | Streptomyces anulatus | - |
? | - |
? | |
| poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Streptomyces anulatus | - |
? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | Rhodococcus equi | latex suspension | (R)-3-hydroxyoctanoate | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | Stenotrophomonas maltophilia | latex suspension | (R)-3-hydroxyoctanoate | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | Pseudomonas fluorescens | latex suspension | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | Pseudomonas alcaligenes | latex suspension | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | Stenotrophomonas maltophilia | latex suspension | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | Streptomyces atratus | latex suspension | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | Streptomyces anulatus | latex suspension | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | Streptomyces omiyaensis | latex suspension | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | Streptomyces beijiangensis | latex suspension | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | Streptomyces pulveraceus | latex suspension | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | Streptomyces roseolus | latex suspension | (R)-3-hydroxyoctanoate + ? | main hydrolysis product is the monomer (R)-3-hydroxyoctanoate | ? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | Stenotrophomonas maltophilia W1 | latex suspension | (R)-3-hydroxyoctanoate | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | Stenotrophomonas maltophilia W1 | latex suspension | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | Pseudomonas alcaligenes SL1 | latex suspension | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | Rhodococcus equi W2 | latex suspension | (R)-3-hydroxyoctanoate | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | Stenotrophomonas maltophilia SL6 | latex suspension | (R)-3-hydroxyoctanoate | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | Stenotrophomonas maltophilia SL6 | latex suspension | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | Streptomyces pulveraceus W3 | latex suspension | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | Streptomyces beijiangensis SL15 | latex suspension | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | Pseudomonas fluorescens GK13 | latex suspension | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxypropionate) + H2O | Streptomyces anulatus | - |
? | - |
? | |
| poly(epsilon-caprolactone) + H2O | Pseudomonas alcaligenes | latex suspension | ? | - |
? | |
| poly(epsilon-caprolactone) + H2O | Streptomyces anulatus | latex suspension | ? | - |
? | |
| poly(epsilon-caprolactone) + H2O | Streptomyces omiyaensis | latex suspension | ? | - |
? | |
| poly(epsilon-caprolactone) + H2O | Streptomyces beijiangensis | latex suspension | ? | - |
? | |
| poly(epsilon-caprolactone) + H2O | Streptomyces pulveraceus | latex suspension | ? | - |
? | |
| poly(epsilon-caprolactone) + H2O | Streptomyces roseolus | latex suspension | ? | - |
? | |
| poly(epsilon-caprolactone) + H2O | Pseudomonas alcaligenes SL1 | latex suspension | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas alcaligenes | - |
- |
- |
| Pseudomonas alcaligenes SL1 | - |
- |
- |
| Pseudomonas fluorescens | - |
- |
- |
| Pseudomonas fluorescens GK13 | - |
- |
- |
| Rhodococcus equi | - |
- |
- |
| Rhodococcus equi W2 | - |
- |
- |
| Stenotrophomonas maltophilia | - |
- |
- |
| Stenotrophomonas maltophilia SL6 | - |
- |
- |
| Stenotrophomonas maltophilia W1 | - |
- |
- |
| Streptomyces anulatus | - |
- |
- |
| Streptomyces anulatus SL11 | - |
- |
- |
| Streptomyces atratus | - |
- |
- |
| Streptomyces atratus SL2 | - |
- |
- |
| Streptomyces beijiangensis | - |
- |
- |
| Streptomyces beijiangensis SL15 | - |
- |
- |
| Streptomyces omiyaensis | - |
isolated from soil, gene phaZSomSO2 | - |
| Streptomyces omiyaensis SO2 CECT 7923 | - |
isolated from soil, gene phaZSomSO2 | - |
| Streptomyces pulveraceus | - |
- |
- |
| Streptomyces pulveraceus W3 | - |
- |
- |
| Streptomyces roseolus | - |
isolated from sludge, gene phaZSroSL3 | - |
| Streptomyces roseolus SL3 CECT 7919 | - |
isolated from sludge, gene phaZSroSL3 | - |
| Streptomyces venezuelae | - |
gene phaZSveSO1 | - |
| Streptomyces venezuelae SO1 CECT 7920 | - |
gene phaZSveSO1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| extracellular enzyme by gel filtration and adsorption chromatography | Streptomyces omiyaensis |
| extracellular enzyme by gel filtration and adsorption chromatography | Streptomyces roseolus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| aerial mycelium | - |
Streptomyces omiyaensis | - |
| aerial mycelium | - |
Streptomyces roseolus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl octanoate + H2O | the enzyme can hydrolyze various substrates for esterases, such as tributyrin and 4-nitrophenyl-alkanoates | Streptomyces roseolus | ? | - |
? | |
| additional information | no activity with poly(ethylene succinate), poly(L-lactide), and poly(epsilon-caprolactone), substrate specificity, overview | Stenotrophomonas maltophilia | ? | - |
? | |
| additional information | no activity with poly(ethylene succinate), poly(L-lactide), poly(3-hydroxybutyrate), and poly(epsilon-caprolactone), substrate specificity, overview | Stenotrophomonas maltophilia | ? | - |
? | |
| additional information | no activity with poly(ethylene succinate), poly(L-lactide), poly(3-hydroxybutyrate), poly(3-hydroxybutyrate-co-3-hydroxyvalerate), and poly(epsilon-caprolactone), substrate specificity, overview | Rhodococcus equi | ? | - |
? | |
| additional information | no activity with poly(ethylene succinate), poly(L-lactide), poly(3-hydroxybutyrate-co-3-hydroxyvalerate), and poly(3-hydroxybutyrate), substrate specificity, overview | Pseudomonas alcaligenes | ? | - |
? | |
| additional information | no activity with poly(ethylene succinate), poly(L-lactide), poly(3-hydroxypropionate), poly(3-hydroxybutyrate), and poly(3-hydroxybutyrate-co-3-hydroxyvalerate), substrate specificity, overview | Pseudomonas fluorescens | ? | - |
? | |
| additional information | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), EC 3.1.1.75, but not with poly(ethylene succinate), poly(L-lactide), and poly(epsilon-caprolactone), substrate specificity in vivo, overview | Streptomyces atratus | ? | - |
? | |
| additional information | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), EC 3.1.1.75, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | Streptomyces anulatus | ? | - |
? | |
| additional information | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), EC 3.1.1.75, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | Streptomyces omiyaensis | ? | - |
? | |
| additional information | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), EC 3.1.1.75, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | Streptomyces roseolus | ? | - |
? | |
| additional information | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate), EC 3.1.1.75, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | Streptomyces beijiangensis | ? | - |
? | |
| additional information | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate), EC 3.1.1.75, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | Streptomyces pulveraceus | ? | - |
? | |
| additional information | no activity with 4-nitrophenyl-hexadecanoate | Streptomyces roseolus | ? | - |
? | |
| additional information | no activity with poly(ethylene succinate), poly(L-lactide), and poly(epsilon-caprolactone), substrate specificity, overview | Stenotrophomonas maltophilia W1 | ? | - |
? | |
| additional information | no activity with poly(ethylene succinate), poly(L-lactide), poly(3-hydroxybutyrate), and poly(epsilon-caprolactone), substrate specificity, overview | Stenotrophomonas maltophilia W1 | ? | - |
? | |
| additional information | no activity with poly(ethylene succinate), poly(L-lactide), poly(3-hydroxybutyrate-co-3-hydroxyvalerate), and poly(3-hydroxybutyrate), substrate specificity, overview | Pseudomonas alcaligenes SL1 | ? | - |
? | |
| additional information | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), EC 3.1.1.75, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | Streptomyces anulatus SL11 | ? | - |
? | |
| additional information | no activity with poly(ethylene succinate), poly(L-lactide), poly(3-hydroxybutyrate), poly(3-hydroxybutyrate-co-3-hydroxyvalerate), and poly(epsilon-caprolactone), substrate specificity, overview | Rhodococcus equi W2 | ? | - |
? | |
| additional information | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), EC 3.1.1.75, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | Streptomyces omiyaensis SO2 CECT 7923 | ? | - |
? | |
| additional information | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), EC 3.1.1.75, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | Streptomyces roseolus SL3 CECT 7919 | ? | - |
? | |
| additional information | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate) and poly(3-hydroxypropionate), EC 3.1.1.75, but not with poly(ethylene succinate), poly(L-lactide), and poly(epsilon-caprolactone), substrate specificity in vivo, overview | Streptomyces atratus SL2 | ? | - |
? | |
| additional information | no activity with poly(ethylene succinate), poly(L-lactide), and poly(epsilon-caprolactone), substrate specificity, overview | Stenotrophomonas maltophilia SL6 | ? | - |
? | |
| additional information | no activity with poly(ethylene succinate), poly(L-lactide), poly(3-hydroxybutyrate), and poly(epsilon-caprolactone), substrate specificity, overview | Stenotrophomonas maltophilia SL6 | ? | - |
? | |
| additional information | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate), EC 3.1.1.75, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | Streptomyces pulveraceus W3 | ? | - |
? | |
| additional information | the enzyme from actinobacteria is active with medium-chain-length polyhydroxyalkanoate with 6 to 14 carbon atoms, e.g. poly(epsilon-caprolactone), as well as with short-chain-length polyhydroxyalkanoates with 3 to 5 carbon atoms like poly(3-hydroxybutyrate), EC 3.1.1.75, but not with poly(ethylene succinate) and poly(L-lactide), substrate specificity in vivo, overview | Streptomyces beijiangensis SL15 | ? | - |
? | |
| additional information | no activity with poly(ethylene succinate), poly(L-lactide), poly(3-hydroxypropionate), poly(3-hydroxybutyrate), and poly(3-hydroxybutyrate-co-3-hydroxyvalerate), substrate specificity, overview | Pseudomonas fluorescens GK13 | ? | - |
? | |
| poly(3-hydroxybutyrate) + H2O | - |
Streptomyces anulatus | ? | - |
? | |
| poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | - |
Streptomyces anulatus | ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Rhodococcus equi | (R)-3-hydroxyoctanoate | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Stenotrophomonas maltophilia | (R)-3-hydroxyoctanoate | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Stenotrophomonas maltophilia W1 | (R)-3-hydroxyoctanoate | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Rhodococcus equi W2 | (R)-3-hydroxyoctanoate | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Stenotrophomonas maltophilia SL6 | (R)-3-hydroxyoctanoate | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Pseudomonas fluorescens | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Pseudomonas alcaligenes | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Streptomyces venezuelae | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Stenotrophomonas maltophilia | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Streptomyces atratus | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Streptomyces anulatus | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Streptomyces omiyaensis | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Streptomyces beijiangensis | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Streptomyces pulveraceus | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Streptomyces roseolus | (R)-3-hydroxyoctanoate + ? | main hydrolysis product is the monomer (R)-3-hydroxyoctanoate | ? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Stenotrophomonas maltophilia W1 | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Pseudomonas alcaligenes SL1 | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Stenotrophomonas maltophilia SL6 | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Streptomyces pulveraceus W3 | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Streptomyces beijiangensis SL15 | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Streptomyces venezuelae SO1 CECT 7920 | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Pseudomonas fluorescens GK13 | (R)-3-hydroxyoctanoate + ? | - |
? | |
| poly(3-hydroxypropionate) + H2O | - |
Streptomyces anulatus | ? | - |
? | |
| poly(epsilon-caprolactone) + H2O | latex suspension | Pseudomonas alcaligenes | ? | - |
? | |
| poly(epsilon-caprolactone) + H2O | latex suspension | Streptomyces anulatus | ? | - |
? | |
| poly(epsilon-caprolactone) + H2O | latex suspension | Streptomyces omiyaensis | ? | - |
? | |
| poly(epsilon-caprolactone) + H2O | latex suspension | Streptomyces beijiangensis | ? | - |
? | |
| poly(epsilon-caprolactone) + H2O | latex suspension | Streptomyces pulveraceus | ? | - |
? | |
| poly(epsilon-caprolactone) + H2O | latex suspension | Streptomyces roseolus | ? | - |
? | |
| poly(epsilon-caprolactone) + H2O | latex suspension | Pseudomonas alcaligenes SL1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 28000, SDS-PAGE | Streptomyces roseolus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mcl PHA depolymerase | - |
Pseudomonas fluorescens |
| mcl PHA depolymerase | - |
Pseudomonas alcaligenes |
| mcl PHA depolymerase | - |
Streptomyces venezuelae |
| mcl PHA depolymerase | - |
Rhodococcus equi |
| mcl PHA depolymerase | - |
Stenotrophomonas maltophilia |
| mcl PHA depolymerase | - |
Streptomyces atratus |
| mcl PHA depolymerase | - |
Streptomyces anulatus |
| mcl PHA depolymerase | - |
Streptomyces omiyaensis |
| mcl PHA depolymerase | - |
Streptomyces beijiangensis |
| mcl PHA depolymerase | - |
Streptomyces pulveraceus |
| mcl PHA depolymerase | - |
Streptomyces roseolus |
| medium-chain-length polyhydroxyalkanoate depolymerase | - |
Pseudomonas fluorescens |
| medium-chain-length polyhydroxyalkanoate depolymerase | - |
Pseudomonas alcaligenes |
| medium-chain-length polyhydroxyalkanoate depolymerase | - |
Streptomyces venezuelae |
| medium-chain-length polyhydroxyalkanoate depolymerase | - |
Rhodococcus equi |
| medium-chain-length polyhydroxyalkanoate depolymerase | - |
Stenotrophomonas maltophilia |
| medium-chain-length polyhydroxyalkanoate depolymerase | - |
Streptomyces atratus |
| medium-chain-length polyhydroxyalkanoate depolymerase | - |
Streptomyces anulatus |
| medium-chain-length polyhydroxyalkanoate depolymerase | - |
Streptomyces omiyaensis |
| medium-chain-length polyhydroxyalkanoate depolymerase | - |
Streptomyces beijiangensis |
| medium-chain-length polyhydroxyalkanoate depolymerase | - |
Streptomyces pulveraceus |
| medium-chain-length polyhydroxyalkanoate depolymerase | - |
Streptomyces roseolus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas alcaligenes |
| 30 | - |
assay at | Streptomyces venezuelae |
| 30 | - |
assay at | Stenotrophomonas maltophilia |
| 30 | - |
assay at | Streptomyces atratus |
| 30 | - |
assay at | Streptomyces anulatus |
| 30 | - |
assay at | Streptomyces omiyaensis |
| 30 | - |
assay at | Streptomyces beijiangensis |
| 30 | - |
assay at | Streptomyces roseolus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Streptomyces roseolus |
| 9.5 | - |
assay at | Pseudomonas fluorescens |
| 9.5 | - |
assay at | Pseudomonas alcaligenes |
| 9.5 | - |
assay at | Streptomyces venezuelae |
| 9.5 | - |
assay at | Rhodococcus equi |
| 9.5 | - |
assay at | Stenotrophomonas maltophilia |
| 9.5 | - |
assay at | Streptomyces atratus |
| 9.5 | - |
assay at | Streptomyces anulatus |
| 9.5 | - |
assay at | Streptomyces omiyaensis |
| 9.5 | - |
assay at | Streptomyces beijiangensis |
| 9.5 | - |
assay at | Streptomyces pulveraceus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10.5 | 60% of maximal activity within this range | Streptomyces roseolus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Streptomyces roseolus | isoelectric focusing | - |
5.2 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview | Pseudomonas fluorescens |
| evolution | enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview | Pseudomonas alcaligenes |
| evolution | enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview | Streptomyces venezuelae |
| evolution | enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview | Rhodococcus equi |
| evolution | enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview | Stenotrophomonas maltophilia |
| evolution | enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview | Streptomyces atratus |
| evolution | enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview | Streptomyces anulatus |
| evolution | enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview | Streptomyces omiyaensis |
| evolution | enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview | Streptomyces beijiangensis |
| evolution | enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview | Streptomyces pulveraceus |
| evolution | enzymes from actinobacteria and gammaproteobacteria seem to belong to different subgroups of the mcl PHA depolymerases, sequence comparisons, overview | Streptomyces roseolus |
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Release 2023.1 (January 2023)
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