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Literature summary for 3.1.1.59 extracted from

  • Stauffer, C.; Shiotsuki, T.; Chan, W.; Hammock, B.D.
    Characterization of the esterase isoenzymes of Ips typographus (Coleoptera, Scolytidae) (1997), Arch. Insect Biochem. Physiol., 34, 203-221.
No PubMed abstract available
Search for more literature for 3.1.1.59

Inhibitors

Inhibitors Comment Organism Structure
3-octylthio-1,1,1-trifluoro-2-propanone more inhibitory than 3-pentylthio-1,1,1-trifluoro-2-propanone and diisopropyl fluorophosphate, moderately potent inhibitor Ips typographus
3-octylthio-1,1,1-trifluoro-2-propanone very potent inhibitor Tenebrio molitor
3-pentylthio-1,1,1-trifluoro-2-propanone less inhibitory than 3-octylthio-1,1,1-trifluoro-2-propanone, more inhibitory than diisopropyl fluorophosphate Ips typographus
3-pentylthio-1,1,1-trifluoro-2-propanone less inhibitory than 3-octylthio-1,1,1-trifluoro-2-propanone, more inhibitory than diisopropyl fluorophosphate Tenebrio molitor
diisopropyl fluorophosphate less inhibitory than 3-octylthio-1,1,1-trifluoro-2-propanone and 3-pentylthio-1,1,1-trifluoro-2-propanone, poor inhibitor Ips typographus
diisopropyl fluorophosphate less inhibitory than 3-octylthio-1,1,1-trifluoro-2-propanone and 3-pentylthio-1,1,1-trifluoro-2-propanone, poor inhibitor Tenebrio molitor
additional information not inhibited by 1% Triton X-100, IC50-values for several inhibitors Ips typographus
additional information IC50-values for several inhibitors Tenebrio molitor
O,O-diethyl-p-nitrophenyl phosphate poor inhibitor Ips typographus
O,O-diethyl-p-nitrophenyl phosphate poor inhibitor Tenebrio molitor
Phenylmethylsulfonylfluoride moderate inhibitor Ips typographus
Phenylmethylsulfonylfluoride weak inhibitor Tenebrio molitor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
juvenile hormone + H2O Ips typographus JHE catabolizes juvenile hormone Juvenile hormone acid + methanol
-
 ?

Organism

Organism UniProt Comment Textmining
Ips typographus
-
-
-
Tenebrio molitor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from pupa, 49fold Tenebrio molitor

Source Tissue

Source Tissue Comment Organism Textmining
adult whole body Ips typographus
-
larva whole body Ips typographus
-
additional information developmental profile of JHE activity with different substrates Ips typographus
-
prepupa
-
 Tenebrio molitor
-
pupa when induced by topical application of juvenile hormone III Ips typographus
-
pupa whole body Tenebrio molitor
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Tenebrio molitor
additional information
-
 specific activities of several developmental stages for the substrates juvenile hormone III, alpha-naphthyl acetate and methyl hexylthio acetothioate Ips typographus
350
-
 purified JHE from pupa, pH 8, 30°C Tenebrio molitor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-naphthyl acetate + H2O
-
 Tenebrio molitor alpha-naphthol + acetate
-
 ?
alpha-naphthyl acetate + H2O specific activities of several developmental stages Ips typographus alpha-naphthol + acetate
-
 ?
juvenile hormone + H2O JHE catabolizes juvenile hormone Ips typographus Juvenile hormone acid + methanol
-
 ?
juvenile hormone III + H2O highest specific activity Tenebrio molitor juvenile hormone III acid + methanol
-
 ?
juvenile hormone III + H2O specific activities of several developmental stages, highest specific activity Ips typographus juvenile hormone III acid + methanol
-
 ?
methyl hexylthio acetothioate + H2O putative surrogate substrate for juvenile hormone Tenebrio molitor ?
-
 ?
methyl hexylthio acetothioate + H2O putative surrogate substrate for juvenile hormone, specific activities of several developmental stages Ips typographus ?
-
 ?

Synonyms

Synonyms Comment Organism
JHE
-
 Tenebrio molitor
JHE
-
 Ips typographus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Tenebrio molitor
30
-
 assay at Ips typographus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Tenebrio molitor
8
-
 assay at Ips typographus

pI Value

Organism Comment pI Value Maximum pI Value
Tenebrio molitor isoelectric focusing
-
 4.34
Ips typographus isoelectric focusing
-
 4.34