Literature summary for 3.1.1.57 extracted from

Maruyama, K.; Shibayama, T.; Ichikawa, A.; Sakou, Y.; Yamada, S.; Sugisaki, H.
Cloning and characterization of the genes encoding enzymes for the protocatechuate meta-degradation pathway of Pseudomonas ochraceae NGJ1 (2004), Biosci. Biotechnol. Biochem., 68, 1434-1441.

Search for more literature for 3.1.1.57

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Pseudomonas straminea

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.025	-	4-oxalomesaconate	pH 6.5, 25°C	Pseudomonas straminea
0.094	-	2-Pyrone-4,6-dicarboxylate	pH 8.5, 25°C	Pseudomonas straminea

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	gel filtration	Pseudomonas straminea
34345	-	1 * 34345, calculated from the deduced amino acid sequence, native mass by gel filtration	Pseudomonas straminea

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-pyrone-4,6-dicarboxylate + H2O	Pseudomonas straminea	involved in the meta-degradation pathway for protocatechuate	4-oxalomesaconate	-	r
2-pyrone-4,6-dicarboxylate + H2O	Pseudomonas straminea NGJ1	involved in the meta-degradation pathway for protocatechuate	4-oxalomesaconate	-	r

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas straminea	-	-	-
Pseudomonas straminea NGJ1	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Pseudomonas straminea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
49.6	-	recombinant protein from Escherichia coli, 25°C	Pseudomonas straminea
495	-	purified recombinant protein from Escherichia coli, 25°C	Pseudomonas straminea

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-pyrone-4,6-dicarboxylate + H2O	-	Pseudomonas straminea	4-oxalomesaconate	-	r
2-pyrone-4,6-dicarboxylate + H2O	involved in the meta-degradation pathway for protocatechuate	Pseudomonas straminea	4-oxalomesaconate	-	r
2-pyrone-4,6-dicarboxylate + H2O	-	Pseudomonas straminea NGJ1	4-oxalomesaconate	-	r
2-pyrone-4,6-dicarboxylate + H2O	involved in the meta-degradation pathway for protocatechuate	Pseudomonas straminea NGJ1	4-oxalomesaconate	-	r

Subunits

Subunits	Comment	Organism
monomer	1 * 34345, calculated from the deduced amino acid sequence, native mass by gel filtration	Pseudomonas straminea

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	reverse reaction	Pseudomonas straminea
8.5	-	forward reaction	Pseudomonas straminea

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Release 2023.1 (January 2023)