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Literature summary for 3.1.1.5 extracted from
- High level expression and characterization of a thermostable lysophospholipase from Thermococcus kodakarensis KOD1 (2012), Extremophiles, 16, 619-625.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ethanol | ethanol increases the enzyme activity to 136% | Thermococcus kodakarensis |  |
| Isopropanol | isopropanol increases the enzyme activity to 133% | Thermococcus kodakarensis | |
| methanol | methanol increases the enzyme activity to 111% | Thermococcus kodakarensis | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Thermococcus kodakarensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | the enzyme is inhibited by 5 mM Cu2+ | Thermococcus kodakarensis | |
| additional information | the enzyme cannot be inhibited by 5 mM EDTA | Thermococcus kodakarensis | |
| Zn2+ | the enzyme is inhibited by 5 mM Zn2+ | Thermococcus kodakarensis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6071 | - |
4-nitrophenyl butyrate | in 50 mM HEPES (pH 8.0), at 85°C | Thermococcus kodakarensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight stimulation | Thermococcus kodakarensis | |
| Ni2+ | slight stimulation | Thermococcus kodakarensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
x * 29000, calculated from amino acid sequence | Thermococcus kodakarensis |
| 32000 | - |
x * 32000, SDS-PAGE | Thermococcus kodakarensis |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| acetonitrile | more than 90% of the enzyme activity retains after incubation with acetonitrile | Thermococcus kodakarensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus kodakarensis | Q5JIE9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Thermococcus kodakarensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl butyrate + H2O | best substrate | Thermococcus kodakarensis | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl caprylate + H2O | - |
Thermococcus kodakarensis | 4-nitrophenol + caprylate | - |
? | |
| 4-nitrophenyl palmitate + H2O | worst substrate | Thermococcus kodakarensis | 4-nitrophenol + palmitate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, SDS-PAGE | Thermococcus kodakarensis |
| ? | x * 29000, calculated from amino acid sequence | Thermococcus kodakarensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LysoPL | - |
Thermococcus kodakarensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Thermococcus kodakarensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 95 | - |
Thermococcus kodakarensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
half-life of 5 h at 85°C and 1 h at 95°C | Thermococcus kodakarensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Thermococcus kodakarensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | - |
Thermococcus kodakarensis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermococcus kodakarensis | calculated fom amino acid sequence | - |
6.4 |
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