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Literature summary for 3.1.1.5 extracted from

  • Yamashita, A.; Tanaka, K.; Kamata, R.; Kumazawa, T.; Suzuki, N.; Koga, H.; Waku, K.; Sugiura, T.
    Subcellular localization and lysophospholipase/transacylation activities of human group IVC phospholipase A2 (cPLA2gamma) (2009), Biochim. Biophys. Acta, 1791, 1011-1022.
    View publication on PubMed
Search for more literature for 3.1.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed as an N-terminal myc-tagged and C-terminal Flag-tagged fusion protein in HeLa or HEK293 cells. C-terminal Flag-tag is inserted into the prenylation site Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information C-terminal Flag-tag is inserted into the prenylation site. Mutant enzyme shows lysophospholipase activity indicating that C-terminal processing is not essential for activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
lysophosphatidic acid inhibition in a dose-dependent manner Homo sapiens
methyl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14- tetraenylphosphonofluoridate
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Homo sapiens 5783
-
mitochondrion enzyme is also located in mitochondria even in the absence of prenylation Homo sapiens 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 SDS-PAGE, Flag-tagged fusion protein Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using membranes and an anti-Flag column Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
C10:0-glycero-3-phosphocholine + H2O rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC Homo sapiens ?
-
 ?
C12:0-glycero-3-phosphocholine + H2O rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC Homo sapiens ?
-
 ?
C14:0-glycero-3-phosphocholine + H2O rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC Homo sapiens ?
-
 ?
C18:0-glycero-3-phosphocholine + H2O rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC Homo sapiens ?
-
 ?
C18:1-glycero-3-phosphocholine + H2O rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC Homo sapiens ?
-
 ?
C18:2-glycero-3-phosphocholine + H2O rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC Homo sapiens ?
-
 ?
C8:0-glycero-3-phosphocholine + H2O rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC Homo sapiens ?
-
 ?
lysophosphatidylethanolamine + H2O
-
 Homo sapiens ?
-
 ?
palmitoyl-glycero-3-phosphocholine + H2O
-
 Homo sapiens glycerophosphocholine + palmitic acid
-
 ?

Synonyms

Synonyms Comment Organism
cPLA2gamma enzyme also possesses transacylation activity: with addition of lysophosphatidic acid/phosphatidic acid, pH above 8, and elevated temperature markedly increases transacylation activity Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens
40
-
 lysophospholipase reaction proceeds even at 0°C and its rate increases with temperature up to roughly 50°C, the optimal temperature is near 40 °C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens