Activating Compound | Comment | Organism | Structure |
---|---|---|---|
carnitine | 10 mM, activation to 121% of control at pH 5.0, LPL1 | Cryptococcus gattii VGIII | |
dithiothreitol | 10 mM, activation to 122% of control at pH 5.0, LPL1 | Cryptococcus gattii VGIII | |
EGTA | 10 mM, 11% activation of phospholipase B activity | Cryptococcus gattii VGIII | |
lysophosphatidylcholine | 0.5 mM, activation of phospholipase B activity to 132% of control | Cryptococcus gattii VGIII | |
palmitoyl carnitine | 0.5 mM, stimulates to 157% of control at pH 7.0, LPL1 | Cryptococcus gattii VGIII | |
palmitoyl carnitine | 1 mM, activation of lysophospholipase activity at pH 7.0 to 239% of control | Cryptococcus gattii VGIII |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 10 mM, 22% inhibition of phospholipase B activity, slight activation of lysophospholipase activity | Cryptococcus gattii VGIII | |
carnitine | 10 mM, 38% inhibition of phospholipase B activity; 10 mM, 9% inhibition at pH 7.0, LPL1 | Cryptococcus gattii VGIII | |
dipalmitoyl phosphatidylcholine | 0.5 mM, 84% inhibition at pH 5.0, 50% inhibition at pH 7.0, LPL 1 | Cryptococcus gattii VGIII | |
dipalmitoylphosphatidylcholine | 0.5 mM, 89% inhibition of lysophospholipase at pH 5.0, 82% inhibition of lysophospholipase at pH 7.0 | Cryptococcus gattii VGIII | |
dithiothreitol | 10 mM, 9% inhibition at pH 7.0, LPL1 | Cryptococcus gattii VGIII | |
EGTA | 10 mM, 15% inhibition at pH 7.0, LPL1 | Cryptococcus gattii VGIII | |
FeCl3 | 10 mM, 39% inhibition at pH 5.0, 38% inhibition at pH 7.0, LPL1; 10 mM, 80% inhibiotion of phospholipase B activity, 17% inhibition of lysophospholipase activity at pH 5.0, 29% in inhibition of lysophospholipase activity at pH 7.0 | Cryptococcus gattii VGIII | |
palmitoyl carnitine | 0.5 mM, 90% inhibition at pH 5.0, LPL1; 1 mM, 64% inhibition of phospholipase B activity, 65% inhibition of lysophospholipase activity at pH 5.0 | Cryptococcus gattii VGIII | |
phosphatidic acid | 0.5 mM, 24% inhibition at pH 5.0, 66% inhibition at pH 7.0, LPL1; 0.5 mM, 58% inhibition of phospholipase B activity, 92% inhibition of lysophospholipase at pH 5.0, 87% inhibition of lysophospholipase at pH 7.0 | Cryptococcus gattii VGIII | |
sphingomyelin | 0.5 MM, 21% inhibition at pH 5.0, 29% inhibition at pH 7.0, LPL1; 0.5 mM, 35% inhibition of phospholipase B activity, 45% inhibition of lysophospholipase at pH 5.0, 28% inhibition of lysophospholipase at pH 7.0 | Cryptococcus gattii VGIII | |
Triton X-100 | 0.1 w/v, 48% inhibition at pH 5.0, LPL1; 0.1% w/v, 85% inhibition of phospholipase B activity, 91% inhibition of lysophospholipase B activity at pH 5.0, 95% inhibition of lysophospholipase B activity at pH 7.0 | Cryptococcus gattii VGIII |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, stimulates to 260% of control at pH 7.0, but not at pH 5.0, LPL1 | Cryptococcus gattii VGIII | |
CaCl2 | 10 mM, 22% inhibition of phospholipase B activity, slight activation of lysophospholipase activity, to 8% at pH 5.0 and 4% at pH 7.0 | Cryptococcus gattii VGIII |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
10 * 66000, isoenzyme LPL1, SDS-PAGE | Cryptococcus gattii VGIII |
220000 | - |
isoenzyme LPL1, gel filtratiin | Cryptococcus neoformans |
275000 | - |
isoenzyme PLB1, gel filtratiin | Cryptococcus gattii VGIII |
670000 | - |
- |
Cryptococcus gattii VGIII |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cryptococcus gattii VGIII | - |
- |
- |
Cryptococcus neoformans | - |
var. grubii, strain H99 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Cryptococcus gattii VGIII |
Purification (Comment) | Organism |
---|---|
isoenzyme LPL1 | Cryptococcus neoformans |
isoenzyme PLB1 | Cryptococcus gattii VGIII |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-oleoyl lysophosphatidylcholine + H2O | 30% of the activity with 1-palmitoyl lysophosphatidylcholine, LPL1 | Cryptococcus gattii VGIII | glycerophosphocholine + oleate | - |
? | |
1-oleoyl-2-lysophosphatidylcholine + H2O | 30% of the activity with 1-palmitoyl lysophosphatidylcholine at pH 5.0, 667% of the activity with 1-palmitoyl lysophosphatidylcholine at pH 7.0 | Cryptococcus gattii VGIII | glycerophosphorylcholine + oleic acid | - |
? | |
1-palmitoyl lysophosphatidylcholine + H2O | preferred substrate, LPL1 | Cryptococcus gattii VGIII | glycerophosphocholine + palmitate | - |
? | |
1-palmitoyl lysophosphatidylcholine + H2O | - |
Cryptococcus gattii VGIII | glycerophosphocholine + palmitic acid | - |
? | |
1-palmitoyl-lysophosphatidylethanolamine + H2O | as active as 1-palmitoyl lysophosphatidylcholine at pH 7.0 | Cryptococcus gattii VGIII | glycerophosphatidylethanolamine + palmitic acid | - |
? | |
1-palmitoyl-lysophosphatidylethanolamine + H2O | 20% of the activity with 1-palmitoyl lysophosphatidylcholine, LPL1 | Cryptococcus gattii VGIII | glycerophosphoethanolamine + palmitate | - |
? | |
1-palmitoyl-sn-glycero-3-phosphoserine + H2O | as active as 1-palmitoyl lysophosphatidylcholine at pH 7.0, 70% of the activity with 1-palmitoyl lysophosphatidylcholine at pH 5.0 | Cryptococcus gattii VGIII | glycerophosphoserine + palmitic acid | - |
? | |
dioleoylphosphatidylcholine + H2O | 2% of the activity with dipalmitoylphosphatidylcholine | Cryptococcus gattii VGIII | ? | - |
? | |
dioleoylphosphatidylethanolamine + H2O | 11% of the activity with dipalmitoylphosphatidylcholine | Cryptococcus gattii VGIII | ? | - |
? | |
dipalmitoylphosphatidylcholine + H2O | - |
Cryptococcus gattii VGIII | ? | - |
? | |
additional information | PLB1 shows phospholipase B activity, lysophospholipase activity and transacylase activity | Cryptococcus gattii VGIII | ? | - |
? | |
additional information | the LPL1 protein only possesses lysophospholipase and transacylase activity | Cryptococcus neoformans | ? | - |
? | |
additional information | the LPL1 protein only possesses lysophospholipase and transacylase activity | Cryptococcus gattii VGIII | ? | - |
? | |
sphingomyelin + H2O | 3% of the activity with dipalmitoylphosphatidylcholine | Cryptococcus gattii VGIII | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 66000, isoenzyme LPL1, SDS-PAGE | Cryptococcus gattii VGIII |
Synonyms | Comment | Organism |
---|---|---|
LPL1 | - |
Cryptococcus neoformans |
LPL1 | - |
Cryptococcus gattii VGIII |
PLB1 enzyme | - |
Cryptococcus gattii VGIII |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Cryptococcus gattii VGIII |
37 | - |
phospholipase B activity, lysophospholipase activity and transacylase activity of PLB1, no activity detectable at higher temperatures | Cryptococcus gattii VGIII |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5 | phospholipase B activity, lysophospholipase activity and transacylase activity of PLB1 | Cryptococcus gattii VGIII |
5 | - |
lysophospholipase activity and transacylase activity, short incubation time (30 s), LPL1 | Cryptococcus gattii VGIII |
5.5 | 8 | lysophospholipase activity, long incubation time (5 min), LPL1 | Cryptococcus gattii VGIII |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.2 | 6 | pH 4.2: about 75% of maximal lysophospholipase activity, pH 6.0: about 75% of maximal lysophospholipase activity, short incubation time (30 s), LPL1 | Cryptococcus gattii VGIII |
7 | - |
no phospholipase B activity, no lysophospholipase activity and no transacylase activity of PLB1 is detected at short incubation time (30 s), at incubation time 5-10 min lysophospholipase activity and transacylase activity is detected (about 5% of the activity at pH 5.0), no phospholipase B activity is detected at incubation time 5-10 min | Cryptococcus gattii VGIII |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cryptococcus gattii VGIII | isoelectric focusing | - |
3.9 |
Cryptococcus gattii VGIII | isoelectric focusing, isoenzyme LPL1 | - |
6.3 |