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Literature summary for 3.1.1.5 extracted from

  • Chen, S.C.; Wright, L.C.; Golding, J.C.; Sorrell, T.C.
    Purification and characterization of secretory phospholipase B, lysophospholipase and lysophospholipase/transacylase from a virulent strain of the pathogenic fungus Cryptococcus neoformans (2000), Biochem. J., 347, 431-439.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol 10 mM, 50% inhibition of phospholipase activity Cryptococcus neoformans
Fe3+ 1 mM, 95% inhibition of phospholipase activity, 80% inhibition of lysophospholipase activity Cryptococcus neoformans
palmitoylcarnitine 10 mM, 70% inhibition of phospholipase activity Cryptococcus neoformans
SDS 0.05%, complete inhibition of phospholipase activity Cryptococcus neoformans
Triton X-100 50% inhibition of lysophospholipase activity Cryptococcus neoformans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.092
-
1-palmitoyl-lysophosphatidylcholine 37°C, pH 4.0 Cryptococcus neoformans
0.19
-
1,2-dipalmitoylphosphatidylcholine 37°C, pH 4.0 Cryptococcus neoformans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Cryptococcus neoformans
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000 232000 native PAGE Cryptococcus neoformans
160000 180000 gel filtration Cryptococcus neoformans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-lysophosphatidylcholine + H2O Cryptococcus neoformans
-
glycerophosphocholine + a carboxylate
-
?
phosphatidylcholine + H2O Cryptococcus neoformans
-
lysophosphatidylcholine + a carboxylate
-
?

Organism

Organism UniProt Comment Textmining
Cryptococcus neoformans
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Cryptococcus neoformans

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, phenyl-Sepharose, Uno Q-1, Superose 12 HR, Superdex 200 Cryptococcus neoformans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.5
-
hydrolysis of 1,2-dipalmitoylphosphatidylcholine Cryptococcus neoformans
559
-
hydrolysis of 1-palmitoyl-lysophosphatidylcholine Cryptococcus neoformans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dioleoylphosphatidylcholine + H2O 129% of activity with 1,2-dipalmitoylphosphatidylcholine Cryptococcus neoformans monooleoyl-lysophosphatidylcholine + oleic acid
-
?
1,2-dioleoylphosphatidylethanolamine + H2O
-
Cryptococcus neoformans monooleoyl-lysophosphatidylethanolamine + oleic acid 33.5% of activity with 1,2-dipalmitoylphosphatidylcholine ?
1,2-dioleoylphosphatidylserine + H2O
-
Cryptococcus neoformans monooleoyl-lysophosphatidylserine + oleic acid 22.5% of activity with 1,2-dipalmitoylphosphatidylcholine ?
1,2-dipalmitoyl-sn-glycero-3-phosphocholine + H2O
-
Cryptococcus neoformans 1-palmitoyl-sn-glycero-3-phosphocholine + palmitate
-
?
1,2-dipalmitoylphosphatidylcholine + H2O
-
Cryptococcus neoformans ?
-
?
1-oleoyl-lysophosphatidylcholine + H2O 194% of activity with 1-palmitoyl-lysophosphatidylcholine Cryptococcus neoformans glycerophosphorylcholine + oleic acid
-
?
1-palmitoyl-2-oleoylphosphatidylcholine + H2O 36% of activity with 1,2-dipalmitoylphosphatidylcholine Cryptococcus neoformans lysophosphatidylcholine + ?
-
?
1-palmitoyl-lysophosphatidylcholine + H2O
-
Cryptococcus neoformans glycerophosphocholine + palmitic acid
-
?
1-palmitoyl-lysophosphatidylethanolamine + H2O 29% of activity with 1-palmitoyl-lysophosphatidylcholine Cryptococcus neoformans glycerophosphatidylethanolamine + palmitic acid
-
?
1-palmitoyl-sn-glycero-3-phosphoserine + H2O 3.8% of activity with 1-palmitoyl-lysophosphatidylcholine Cryptococcus neoformans glycerophosphoserine + palmitic acid
-
?
2-lysophosphatidylcholine + H2O
-
Cryptococcus neoformans glycerophosphocholine + a carboxylate
-
?
lysophosphatidylinositol + H2O 78% of activity with 1-palmitoyl-lysophosphatidylcholine Cryptococcus neoformans glycerophosphatidylinositol + a carboxylate
-
?
phosphatidylcholine + H2O
-
Cryptococcus neoformans lysophosphatidylcholine + a carboxylate
-
?
phosphatidylglycerol + H2O 94.2% of activity with 1,2-dipalmitoylphosphatidylcholine Cryptococcus neoformans lysophosphatidylglycerol + a carboxylate
-
?
phosphatidylinositol + H2O 15.6% of activity with 1,2-dipalmitoylphosphatidylcholine Cryptococcus neoformans lysophophatidylinositol + a carboxylate
-
?
platelet activating factor + H2O 8.9% of activity with 1,2-dipalmitoylphosphatidylcholine Cryptococcus neoformans platelet activating factor + a carboxylate
-
?

Subunits

Subunits Comment Organism
? x * 70000-90000, SDS-PAGE Cryptococcus neoformans

Synonyms

Synonyms Comment Organism
phospholipase B
-
Cryptococcus neoformans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
lysophospholipase activity Cryptococcus neoformans
37
-
phospholipase activity Cryptococcus neoformans
40
-
transacylase activity Cryptococcus neoformans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
45% loss of lysophospholipase and transacylase activity, complete loss of phospholipase activity Cryptococcus neoformans
60
-
complete loss of lysophospholipase and transacylase activity Cryptococcus neoformans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
hydrolysis of phosphatidylcholin and lysophosphatidylcholin Cryptococcus neoformans

pH Range

pH Minimum pH Maximum Comment Organism
2.5 6 approx. 50% of maximal phospholipase activity at pH 3.0 and pH 5.0, respectively Cryptococcus neoformans
2.5 7 50-60% of maximal lysophospholipase activity at pH 3.0 and pH 5.5, respectively Cryptococcus neoformans

pI Value

Organism Comment pI Value Maximum pI Value
Cryptococcus neoformans 2D-electrophoresis
-
3.5