General Stability | Organism |
---|---|
unstable after deglycosylation with endoglycosidase H | Rhodotorula toruloides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dichloroisocoumarin | - |
Rhodotorula toruloides | |
dimethyl phosphite | - |
Rhodotorula toruloides | |
PMSF | - |
Rhodotorula toruloides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
51.8 | - |
cephalosporin C | - |
Rhodotorula toruloides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61315 | - |
x * 61315, calculation from amino acid sequence | Rhodotorula toruloides |
80000 | - |
x * 80000, SDS-PAGE | Rhodotorula toruloides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Rhodotorula toruloides | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | glycoprotein, sugar moieties may play a role in stabilizing the native structure of the enzyme | Rhodotorula toruloides |
Purification (Comment) | Organism |
---|---|
- |
Rhodotorula toruloides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodotorula toruloides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O | - |
Bacillus subtilis | deacetylcephalosporin C + acetate | - |
? | |
cephalosporin C + H2O | - |
Rhodotorula toruloides | deacetylcephalosporin C + acetate | - |
? | |
deacetyl-7-aminocephalosporanic acid + ethylene glycol diacetate | - |
Bacillus subtilis | 7-aminocephalosporanic acid + ? | - |
? | |
deacetyl-7-aminocephalosporanic acid + ethylene glycol diacetate | - |
Rhodotorula toruloides | 7-aminocephalosporanic acid + ? | - |
? | |
deacetyl-7-aminocephalosporanic acid + isopropenyl acetate | - |
Bacillus subtilis | 7-aminocephalosporanic acid + ? | - |
? | |
p-nitrophenyl acetate + H2O | - |
Bacillus subtilis | 4-nitrophenol + acetate | - |
? | |
p-nitrophenyl acetate + H2O | - |
Rhodotorula toruloides | 4-nitrophenol + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, SDS-PAGE | Rhodotorula toruloides |
? | x * 61315, calculation from amino acid sequence | Rhodotorula toruloides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Rhodotorula toruloides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Bacillus subtilis |
25 | - |
unstable above | Rhodotorula toruloides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Rhodotorula toruloides |