Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.41 extracted from

  • Abraham, E.P.; Fawcett, P.
    Cephalosporin acetylesterase (citrus) (1975), Methods Enzymol., 43, 728-731.
    View publication on PubMed
Search for more literature for 3.1.1.41

General Stability

General Stability Organism
dialysis against water causes 50% loss of activity Citrus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.7
-
 cephalosporin C
-
 Citrus sp.
8.6
-
 cephalotin
-
 Citrus sp.
30 32 Triacetin
-
 Citrus sp.

Organism

Organism UniProt Comment Textmining
Citrus sp.
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit peel Citrus sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-aminocephalosporanic acid + H2O
-
 Citrus sp. acetate + deacetyl-7-aminocephalosporanic acid
-
 ?
cephalosporin C + H2O
-
 Citrus sp. deacetylcephalosporin C + acetate
-
 ?
cephalothin + H2O
-
 Citrus sp. acetate + deacetylcephalothin
-
 ?
triacetin + H2O
-
 Citrus sp. ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, 90% loss of activity Citrus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 hydrolysis of monoacetin Citrus sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7 10% of maximal activity at pH 5.5 and at pH 7.0 Citrus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 rapid inactivation at or below Citrus sp.
5 8.3 25°C, stable Citrus sp.