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Literature summary for 3.1.1.41 extracted from
- Physical properties and kinetic behavior of a cephalosporin acetylesterase produced by Bacillus subtilis (1975), Appl. Microbiol., 30, 413-419.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetate | - |
Bacillus subtilis |  |
| deacetyl-7-aminocephalosporanic acid | - |
Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.8 | - |
7-aminocephalosporanic acid | - |
Bacillus subtilis | |
| 8.3 | - |
cephalothin | - |
Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 190000 | - |
gel filtration | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Bacillus subtilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 25°C, 1 month, more than 80% of the activity is retained | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-aminocephalosporanic acid + H2O | - |
Bacillus subtilis | acetate + deacetyl-7-aminocephalosporanic acid | - |
? | |
| cephalothin + H2O | - |
Bacillus subtilis | acetate + deacetylcephalothin | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | - |
Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | 20°C: about 40% of maximal activity, 60°C: about 10% of maximal activity | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
1 month, less than 20% loss of activity | Bacillus subtilis |
| 70 | - |
salt-free enzyme, 5 min, 50% loss of activity | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Bacillus subtilis |
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