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Literature summary for 3.1.1.4 extracted from

  • Shrestha, S.; Kim, Y.
    Biochemical characteristics of immune-associated phospholipase A2 and its inhibition by an entomopathogenic bacterium, Xenorhabdus nematophila (2010), J. Microbiol., 47, 774-782.
    View publication on PubMed
Search for more literature for 3.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Tribolium castaneum

Inhibitors

Inhibitors Comment Organism Structure
bromophenacyl bromide specific sPLA2 inhibitor Tribolium castaneum
dithiothreitol
-
 Tribolium castaneum
EGTA almost complete inhibition at 0.06 mM EGTA Tribolium castaneum
additional information sPLA2 is not inhibited by methylarachidonyl fluorophosphonate Tribolium castaneum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulation of activity in the presence of 0.6 mM Ca2+ Tribolium castaneum

Organism

Organism UniProt Comment Textmining
Tribolium castaneum C0LTQ2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-hexadecanoyl-2-(1-pyrenedecanoyl)-sn-glycerol-3-phosphatidyl choline + H2O
-
 Tribolium castaneum ?
-
 ?

Synonyms

Synonyms Comment Organism
secretory phospholipase A2
-
 Tribolium castaneum
sPLA2
-
 Tribolium castaneum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Tribolium castaneum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Tribolium castaneum

Expression

Organism Comment Expression
Tribolium castaneum Xenorhabdus nematophila induces an immunosuppression of target insects by inhibiting phospholipase A2 activity down