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Literature summary for 3.1.1.4 extracted from

  • Ayilavarapu, S.; Kantarci, A.; Fredman, G.; Turkoglu, O.; Omori, K.; Liu, H.; Iwata, T.; Yagi, M.; Hasturk, H.; Van Dyke, T.E.
    Diabetes-induced oxidative stress is mediated by Ca2+-independent phospholipase A2 in neutrophils (2010), J. Immunol., 184, 1507-1515.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.4

Inhibitors

Inhibitors Comment Organism Structure
bromoenol lactone specific iPLA2 inhibitor, which completely suppresses fMLP-induced generation of superoxide. Completely blocks superoxide generation in neutrophils from healthy and diabetic subjects. Exogeneous arachidonic acid rescues neutrophil superoxide inhibition by partly restoring NADPH oxidase activity, resulting in superoxide generation Homo sapiens
pyrrolidine-1 specific cPLA2 inhibitor, does not inhibit the superoxide generation in healthy neutrophils Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane iPLA2 Homo sapiens 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
 x * 85000, iPLA2, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
neutrophil iPLA2 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonyl thio-PC + H2O
-
 Homo sapiens arachidonic acid + (7R)-4-hydroxy-N,N,N-trimethyl-7-sulfanyl-3,5,9-trioxa-4-phosphapentacosan-1-aminium 4-oxide
-
 ?

Subunits

Subunits Comment Organism
? x * 85000, iPLA2, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
Ca2+-independent phospholipase A2
-
 Homo sapiens
cPLA2
-
 Homo sapiens
iPLA2
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens transfection with iPLA2 siRNA results in ca. 50% reduction in mRNA levels of iPLA2 down
Homo sapiens iPLA2 mRNA expression is increased in neutrophils from people with diabetes and mediates superoxide generation up

General Information

General Information Comment Organism
malfunction small interfering RNA knockdown of iPLA2 inhibits superoxide generation by neutrophils Homo sapiens
physiological function role of iPLA2 in enhanced superoxide generation in neutrophils from people with diabetes mellitus, iPLA2 presents an alternate pathway independent of protein kinase C and phosphatidic acid phosphohydrolase-1 hydrolase signaling. The iPLA2 does not regulate cell apoptosis or cell death in neutrophils, iPLA2 regulates superoxide generation independently of p47phox or pleckstrin phosphorylation. The iPLA2-derived arachidonic acid activates NAPDH oxidase to generate superoxide anion Homo sapiens