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Literature summary for 3.1.1.4 extracted from

  • Hu, P.; Sun, L.; Zhu, Z.Q.; Hou, X.W.; Wang, S.; Yu, S.S.; Wang, H.L.; Zhang, P.; Wang, M.; Niu, L.W.; Teng, M.K.; Ruan, D.Y.
    Crystal structure of Natratoxin, a novel snake secreted phospholipaseA2 neurotoxin from Naja atra venom inhibiting A-type K+ currents (2008), Proteins, 72, 673-683.
    View publication on PubMed
Search for more literature for 3.1.1.4

Crystallization (Commentary)

Crystallization (Comment) Organism
purified native enzyme, hanging drop vapour diffusion method, 8°C, X-ray diffraction structure determination and analysis at 2.2 A resolution, molecular replacement method Naja atra

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secereted enzyme in venom Naja atra
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ does only slightly alter the effects of the enzyme on K+ currents, overview Naja atra

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Naja atra the enzyme in venom effectively inhibits A-type K+ currents in mice and cause alterations of channel gating characters, such as the shifts of steadystate activation and inactivation curves to hyperpolarization direction and changes of V1/2 and slope factor, the effects are independent of the enzymatic activity as phospholipase, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Naja atra
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from venom by cation and anion exchange chromatography Naja atra

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Naja atra
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme in venom effectively inhibits A-type K+ currents in mice and cause alterations of channel gating characters, such as the shifts of steadystate activation and inactivation curves to hyperpolarization direction and changes of V1/2 and slope factor, the effects are independent of the enzymatic activity as phospholipase, overview Naja atra ?
-
 ?

Synonyms

Synonyms Comment Organism
natratoxin
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 Naja atra
secreted phospholipase A2
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 Naja atra
secreted phospholipaseA2 neurotoxin
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 Naja atra
sPLA2
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 Naja atra

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 assay at Naja atra