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Literature summary for 3.1.1.4 extracted from

  • Duncan, R.E.; Sarkadi-Nagy, E.; Jaworski, K.; Ahmadian, M.; Sul, H.S.
    Identification and functional characterization of adipose-specific phospholipase A2 (AdPLA) (2008), J. Biol. Chem., 283, 25428-25436.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of His-tagged and/or fluoresence-tagged phospholipase A2 in COS-7 cells, perinuclear region, membranes and cytosol, or 3T3-L1 preadipocytic cells Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
7,7-dimethyl-5,8-eicosadienoic acid
-
 Rattus norvegicus
arachidonyl trifluoromethyl ketone i.e. AACOCF3 Rattus norvegicus
methyl arachidonyl fluorophosphate i.e. MAFP, an inhibitor of cysteine-dependent phospholipases Rattus norvegicus
additional information no inhibition of AdPLA and sPLA2 by bromoenolactone Rattus norvegicus
thioetheramide
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 1-palmitoyl-2-linoleoyl-phosphatidylethanolamine pH 8.0, 25°C, recombinant enzyme Rattus norvegicus
0.0073
-
 1-palmitoyl-2-linoleoyl-phosphatidylserine pH 8.0, 25°C, recombinant enzyme Rattus norvegicus
0.0082
-
 1-palmitoyl-2-arachidonyl-phosphatidylcholine pH 8.0, 25°C, recombinant enzyme Rattus norvegicus
0.0116
-
 1-palmitoyl-2-linoleoyl-phosphatidic acid pH 8.0, 25°C, recombinant enzyme Rattus norvegicus
0.0175
-
 1,2-dilinoleoyl-phosphatidylcholine pH 8.0, 25°C, recombinant enzyme Rattus norvegicus
0.0186
-
 1-palmitoyl-2-linoleoyl-phosphatidylcholine pH 8.0, 25°C, recombinant enzyme Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol AdPLA Rattus norvegicus 5829
-
membrane the adipose-specific phospholipase A2 AdPLA is membrane-associated Rattus norvegicus 16020
-
additional information subcellular localization of AdPLA, overview Rattus norvegicus
-
-
perinuclear space AdPLA Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the adipose-specific phospholipase A2 is activated by Ca2+, but it is not essential for activity Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus phospholipases A2 catalyze hydrolysis of fatty acids from the sn-2 position of phospholipids ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 female Zucker rats
-

Source Tissue

Source Tissue Comment Organism Textmining
colon
-
 Rattus norvegicus
-
gonad
-
 Rattus norvegicus
-
heart
-
 Rattus norvegicus
-
kidney
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-
lung
-
 Rattus norvegicus
-
additional information expression profiles of isozymes iPLA2beta, sPLA2alpha, and AdPLA, overview Rattus norvegicus
-
muscle
-
 Rattus norvegicus
-
skin
-
 Rattus norvegicus
-
spleen
-
 Rattus norvegicus
-
white adipose tissue AdPLA is highly expressed specifically in white adipose tissue and is induced during preadipocyte differentiation into adipocytes, overview Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dilinoleoyl-phosphatidylcholine + H2O
-
 Rattus norvegicus 1-linoleoyl-sn-glycero-3-phosphocholine + linoleic acid
-
 ?
1,2-dilinoleoyl-phosphatidylcholine + H2O
-
 Rattus norvegicus 1-linoleoyl-glycerophosphorylcholine + linoleic acid
-
 ?
1-palmitoyl-2-arachidonyl-phosphatidylcholine + H2O
-
 Rattus norvegicus 1-palmitoyl-sn-glycero-3-phosphocholine + arachidonic acid
-
 ?
1-palmitoyl-2-linoleoyl-phosphatidic acid + H2O
-
 Rattus norvegicus 1-palmitoyl-sn-glycero-3-phosphate + linoleic acid
-
 ?
1-palmitoyl-2-linoleoyl-phosphatidylcholine + H2O
-
 Rattus norvegicus 1-palmitoyl-sn-glycero-3-phosphocholine + linoleic acid
-
 ?
1-palmitoyl-2-linoleoyl-phosphatidylethanolamine + H2O
-
 Rattus norvegicus 1-palmitoyl-sn-glycerophosphoethanolamine + linoleic acid
-
 ?
1-palmitoyl-2-linoleoyl-phosphatidylinositol + H2O
-
 Rattus norvegicus 1-palmitoyl-3-phosphoinositol + linoleic acid
-
 ?
1-palmitoyl-2-linoleoyl-phosphatidylserine + H2O
-
 Rattus norvegicus 1-palmitoyl-sn-glycero-3-phosphoserine + linoleic acid
-
 ?
hen egg phosphatidylcholine + H2O
-
 Rattus norvegicus ?
-
 ?
additional information phospholipases A2 catalyze hydrolysis of fatty acids from the sn-2 position of phospholipids Rattus norvegicus ?
-
 ?
additional information AdPLA is most likely a His-Cys PLA2, His23 and Cys113 are critical for activity of AdPLA, catalytic mechanism, overview. AdPLA has phospholipase activity but lacks acyltransferase activity Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
More AdPLA contains no characteristic serine lipase motif, i.e. GXSXG or GXSGS Rattus norvegicus

Synonyms

Synonyms Comment Organism
AdPLA
-
 Rattus norvegicus
cPLA2alpha
-
 Rattus norvegicus
specific phospholipase A2
-
 Rattus norvegicus
sPLA2
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Rattus norvegicus