Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.4 extracted from

  • Correa, L.C.; Marchi-Salvador, D.P.; Cintra, A.C.; Sampaio, S.V.; Soares, A.M.; Fontes, M.R.
    Crystal structure of a myotoxic Asp49-phospholipase A2 with low catalytic activity: Insights into Ca2+-independent catalytic mechanism (2008), Biochim. Biophys. Acta, 1784, 591-599.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
lyophilized Asp49-PLA2, mixing of 0.001 ml of solution containing 12 mg/ml of protein with 0.001 ml of precipitant solution containing 20% v/v 2-propanol, 13% w/v PEG 4000, and 0.1 M sodium citrate, pH 5.6, hanging drop vapor diffusion method, 2 months, 18°C, X-ray diffraction structure determination and analysis at 2.19 A resolution, molecular replacement Bothrops jararacussu

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bothrops jararacussu
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme is Ca2+-independent Bothrops jararacussu

Organism

Organism UniProt Comment Textmining
Bothrops jararacussu P45881
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme from venom by gel filtration and ion exchange chromatography Bothrops jararacussu

Reaction

Reaction Comment Organism Reaction ID
phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate Ca2+-independent catalytic mechanism, overview Bothrops jararacussu

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Bothrops jararacussu
-

Subunits

Subunits Comment Organism
dimer dimeric structure with seven disulfide bridges in each monomer, quaternary structure of BthTX-II, structure comparisons, overview Bothrops jararacussu

Synonyms

Synonyms Comment Organism
Asp49-PLA2
-
Bothrops jararacussu
BthTX-II
-
Bothrops jararacussu
myotoxic Asp49-phospholipase A2
-
Bothrops jararacussu