Crystallization (Comment) | Organism |
---|---|
lyophilized Asp49-PLA2, mixing of 0.001 ml of solution containing 12 mg/ml of protein with 0.001 ml of precipitant solution containing 20% v/v 2-propanol, 13% w/v PEG 4000, and 0.1 M sodium citrate, pH 5.6, hanging drop vapor diffusion method, 2 months, 18°C, X-ray diffraction structure determination and analysis at 2.19 A resolution, molecular replacement | Bothrops jararacussu |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bothrops jararacussu | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is Ca2+-independent | Bothrops jararacussu |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bothrops jararacussu | P45881 | - |
- |
Purification (Comment) | Organism |
---|---|
enzyme from venom by gel filtration and ion exchange chromatography | Bothrops jararacussu |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate | Ca2+-independent catalytic mechanism, overview | Bothrops jararacussu |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Bothrops jararacussu | - |
Subunits | Comment | Organism |
---|---|---|
dimer | dimeric structure with seven disulfide bridges in each monomer, quaternary structure of BthTX-II, structure comparisons, overview | Bothrops jararacussu |
Synonyms | Comment | Organism |
---|---|---|
Asp49-PLA2 | - |
Bothrops jararacussu |
BthTX-II | - |
Bothrops jararacussu |
myotoxic Asp49-phospholipase A2 | - |
Bothrops jararacussu |