Activating Compound | Comment | Organism | Structure |
---|---|---|---|
12-O-tetradecanoylphorbol-13-acetate | a protein kinase C agonist, has no effect on Prdx6 expression but leads to about 75% increase in aiPLA2 activity | Homo sapiens | |
12-O-tetradecanoylphorbol-13-acetate | a protein kinase C agonist, has no effect on Prdx6 expression but leads to about 75% increase in aiPLA2 activity | Rattus norvegicus | |
additional information | the JNK inhibitor has no effect on TPA-induced PLA2 activity, either in the intact cells or cell lysate | Homo sapiens | |
additional information | the JNK inhibitor has no effect on TPA-induced PLA2 activity, either in the intact cells or cell lysate | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant enzymes | Homo sapiens |
expression of untagged enzyme | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
T177A | the mutation of Thr177 abolishes protein phosphorylation and the increase in MAP kinase-mediated activity of the mutant | Homo sapiens |
T177E | the mutation of Thr177 abolishes protein phosphorylation and the increase in MAP kinase-mediated activity of the mutant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hexadecyl-3-trifluorethylglycero-sn-2-phospho-methanol | i.e. MJ33 | Homo sapiens | |
hexadecyl-3-trifluorethylglycero-sn-2-phospho-methanol | i.e. MJ33 | Rattus norvegicus | |
additional information | SB202190 and PD98059 abolish the enzyme activation by phosphorylation through protein kinase C by specific inhibition of PKC | Homo sapiens | |
additional information | SB202190 and PD98059 abolish the enzyme activation by phosphorylation through protein kinase C by specific inhibition of PKC | Rattus norvegicus | |
surfactant protein A | - |
Homo sapiens | |
surfactant protein A | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | independence of Ca2+ | Homo sapiens | |
additional information | independence of Ca2+ | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | peroxiredoxin 6 is a bifunctional protein with both GSH peroxidase and phospholipase A2 activities, and is responsible for the metabolism of lung surfactant phospholipids. Prdx6 has a major role in degradation of internalized dipalmitoyl phosphatidylcholine as well as in the remodeling of PC through the deacylation-reacylation pathway by lung granular pneumocytes, i.e. alveolar epithelial type II cells. Mitogen-activated protein kinase-mediated phosphorylation of peroxiredoxin 6 regulates its phospholipase A2 activity, overview | ? | - |
? | |
additional information | Rattus norvegicus | peroxiredoxin 6 is a bifunctional protein with both GSH peroxidase and phospholipase A2 activities, and is responsible for the metabolism of lung surfactant phospholipids. Prdx6 has a major role in degradation of internalized dipalmitoyl phosphatidylcholine as well as in the remodeling of PC through the deacylation-reacylation pathway by lung granular pneumocytes, i.e. alveolar epithelial type II cells. Mitrogen-activated protein kinase-mediated phosphorylation of peroxiredoxin 6 regulates its phospholipase A2 activity, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
Sprague-Dawley male rats | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the aiPLA2 is activated by phosphorylation, specifically at Thr177, through protein kinase C by about 75%, several active isoforms of Erk and p38 phosphorylate Prdx6 resulting in a 11fold increase in aiPLA2 activity | Homo sapiens |
phosphoprotein | the aiPLA2 is activated by phosphorylation, specifically at Thr177, through protein kinase C by about 75%, several active isoforms of Erk and p38 phosphorylate Prdx6 resulting in a 11fold increase in aiPLA2 activity | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme by nickel affinity chromatography and gel filtration | Homo sapiens |
recombinant untagged enzyme by ion-exchange chromatography and gel filtration | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
alveolar type II cell | - |
Homo sapiens | - |
alveolar type II cell | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dipalmitoyl-sn-glycero-3-phosphorylcholine + H2O | - |
Homo sapiens | ? | - |
? | |
1,2-dipalmitoyl-sn-glycero-3-phosphorylcholine + H2O | - |
Rattus norvegicus | 1-palmitoyl-sn-glycero-3-phosphorylcholine + palmitate | - |
? | |
additional information | peroxiredoxin 6 is a bifunctional protein with both GSH peroxidase and phospholipase A2 activities, and is responsible for the metabolism of lung surfactant phospholipids. Prdx6 has a major role in degradation of internalized dipalmitoyl phosphatidylcholine as well as in the remodeling of PC through the deacylation-reacylation pathway by lung granular pneumocytes, i.e. alveolar epithelial type II cells. Mitogen-activated protein kinase-mediated phosphorylation of peroxiredoxin 6 regulates its phospholipase A2 activity, overview | Homo sapiens | ? | - |
? | |
additional information | peroxiredoxin 6 is a bifunctional protein with both GSH peroxidase and phospholipase A2 activities, and is responsible for the metabolism of lung surfactant phospholipids. Prdx6 has a major role in degradation of internalized dipalmitoyl phosphatidylcholine as well as in the remodeling of PC through the deacylation-reacylation pathway by lung granular pneumocytes, i.e. alveolar epithelial type II cells. Mitrogen-activated protein kinase-mediated phosphorylation of peroxiredoxin 6 regulates its phospholipase A2 activity, overview | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aiPLA2 | - |
Homo sapiens |
aiPLA2 | - |
Rattus norvegicus |
peroxiredoxin 6 | - |
Homo sapiens |
peroxiredoxin 6 | - |
Rattus norvegicus |
Prdx6 | - |
Homo sapiens |
Prdx6 | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
7.4 | - |
assay at | Rattus norvegicus |