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Literature summary for 3.1.1.4 extracted from

  • Wu, Y.; Feinstein, S.I.; Manevich, Y.; Chowdhury, I.; Pak, J.H.; Kazi, A.; Dodia, C.; Speicher, D.W.; Fisher, A.B.
    Mitrogen-activated protein kinase-mediated phosphorylation of peroxiredoxin 6 regulates its phospholipase A2 activity (2009), Biochem. J., 419, 669-679.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
12-O-tetradecanoylphorbol-13-acetate a protein kinase C agonist, has no effect on Prdx6 expression but leads to about 75% increase in aiPLA2 activity Homo sapiens
12-O-tetradecanoylphorbol-13-acetate a protein kinase C agonist, has no effect on Prdx6 expression but leads to about 75% increase in aiPLA2 activity Rattus norvegicus
additional information the JNK inhibitor has no effect on TPA-induced PLA2 activity, either in the intact cells or cell lysate Homo sapiens
additional information the JNK inhibitor has no effect on TPA-induced PLA2 activity, either in the intact cells or cell lysate Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and mutant enzymes Homo sapiens
expression of untagged enzyme Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
T177A the mutation of Thr177 abolishes protein phosphorylation and the increase in MAP kinase-mediated activity of the mutant Homo sapiens
T177E the mutation of Thr177 abolishes protein phosphorylation and the increase in MAP kinase-mediated activity of the mutant Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
hexadecyl-3-trifluorethylglycero-sn-2-phospho-methanol i.e. MJ33 Homo sapiens
hexadecyl-3-trifluorethylglycero-sn-2-phospho-methanol i.e. MJ33 Rattus norvegicus
additional information SB202190 and PD98059 abolish the enzyme activation by phosphorylation through protein kinase C by specific inhibition of PKC Homo sapiens
additional information SB202190 and PD98059 abolish the enzyme activation by phosphorylation through protein kinase C by specific inhibition of PKC Rattus norvegicus
surfactant protein A
-
Homo sapiens
surfactant protein A
-
Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information independence of Ca2+ Homo sapiens
additional information independence of Ca2+ Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens peroxiredoxin 6 is a bifunctional protein with both GSH peroxidase and phospholipase A2 activities, and is responsible for the metabolism of lung surfactant phospholipids. Prdx6 has a major role in degradation of internalized dipalmitoyl phosphatidylcholine as well as in the remodeling of PC through the deacylation-reacylation pathway by lung granular pneumocytes, i.e. alveolar epithelial type II cells. Mitogen-activated protein kinase-mediated phosphorylation of peroxiredoxin 6 regulates its phospholipase A2 activity, overview ?
-
?
additional information Rattus norvegicus peroxiredoxin 6 is a bifunctional protein with both GSH peroxidase and phospholipase A2 activities, and is responsible for the metabolism of lung surfactant phospholipids. Prdx6 has a major role in degradation of internalized dipalmitoyl phosphatidylcholine as well as in the remodeling of PC through the deacylation-reacylation pathway by lung granular pneumocytes, i.e. alveolar epithelial type II cells. Mitrogen-activated protein kinase-mediated phosphorylation of peroxiredoxin 6 regulates its phospholipase A2 activity, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
Sprague-Dawley male rats
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the aiPLA2 is activated by phosphorylation, specifically at Thr177, through protein kinase C by about 75%, several active isoforms of Erk and p38 phosphorylate Prdx6 resulting in a 11fold increase in aiPLA2 activity Homo sapiens
phosphoprotein the aiPLA2 is activated by phosphorylation, specifically at Thr177, through protein kinase C by about 75%, several active isoforms of Erk and p38 phosphorylate Prdx6 resulting in a 11fold increase in aiPLA2 activity Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme by nickel affinity chromatography and gel filtration Homo sapiens
recombinant untagged enzyme by ion-exchange chromatography and gel filtration Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
alveolar type II cell
-
Homo sapiens
-
alveolar type II cell
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dipalmitoyl-sn-glycero-3-phosphorylcholine + H2O
-
Homo sapiens ?
-
?
1,2-dipalmitoyl-sn-glycero-3-phosphorylcholine + H2O
-
Rattus norvegicus 1-palmitoyl-sn-glycero-3-phosphorylcholine + palmitate
-
?
additional information peroxiredoxin 6 is a bifunctional protein with both GSH peroxidase and phospholipase A2 activities, and is responsible for the metabolism of lung surfactant phospholipids. Prdx6 has a major role in degradation of internalized dipalmitoyl phosphatidylcholine as well as in the remodeling of PC through the deacylation-reacylation pathway by lung granular pneumocytes, i.e. alveolar epithelial type II cells. Mitogen-activated protein kinase-mediated phosphorylation of peroxiredoxin 6 regulates its phospholipase A2 activity, overview Homo sapiens ?
-
?
additional information peroxiredoxin 6 is a bifunctional protein with both GSH peroxidase and phospholipase A2 activities, and is responsible for the metabolism of lung surfactant phospholipids. Prdx6 has a major role in degradation of internalized dipalmitoyl phosphatidylcholine as well as in the remodeling of PC through the deacylation-reacylation pathway by lung granular pneumocytes, i.e. alveolar epithelial type II cells. Mitrogen-activated protein kinase-mediated phosphorylation of peroxiredoxin 6 regulates its phospholipase A2 activity, overview Rattus norvegicus ?
-
?

Synonyms

Synonyms Comment Organism
aiPLA2
-
Homo sapiens
aiPLA2
-
Rattus norvegicus
peroxiredoxin 6
-
Homo sapiens
peroxiredoxin 6
-
Rattus norvegicus
Prdx6
-
Homo sapiens
Prdx6
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens
7.4
-
assay at Rattus norvegicus