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Literature summary for 3.1.1.4 extracted from
- Purification and characterization of a low molecular weight multifunctional cytotoxic phospholipase A2 from Russells viper venom (2007), J. Chromatogr. B, 845, 232-243.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EGTA | - |
Daboia russelii russelii |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | absolutely requird for the PLA2 activity of RVV-7 | Daboia russelii russelii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 6500 | 7000 | RVV-7, native PAGE | Daboia russelii russelii |
| 7201 | 7224 | RVV-7, mass spectrometry | Daboia russelii russelii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Daboia russelii russelii | the PLA2, i.e. RVV-7, is the basic toxin of Russells viper venom, it shows cytotoxic activity with B16F10 melanoma cells, it inhibits trypsin, but not chymotrypsin or bromelain, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Daboia russelii russelii | - |
Indian Russell's viper | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme RVV-7 from venom purified to homogeneity after partial unfolding by 4 M urea followed by ultrafiltration and dialysis | Daboia russelii russelii |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate | the active site His48 is conserved in all venom PLA2s | Daboia russelii russelii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Daboia russelii russelii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
RVV-7 cytotoxic activity versus B16F10 melanoma cells, overview | Daboia russelii russelii |
| 0.121 | - |
purified enzyme | Daboia russelii russelii |
| 0.347 | - |
crude venom preparation | Daboia russelii russelii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-dimyristoyl-sn-glycero-3 phosphocholine + H2O | - |
Daboia russelii russelii | 1-myristoyl-phosphorylcholine + myristic acid | - |
? | |
| additional information | the PLA2, i.e. RVV-7, is the basic toxin of Russells viper venom, it shows cytotoxic activity with B16F10 melanoma cells, it inhibits trypsin, but not chymotrypsin or bromelain, overview | Daboia russelii russelii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 7201-7224, mass spetrometry | Daboia russelii russelii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RVV-7 | - |
Daboia russelii russelii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Daboia russelii russelii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
3 h, purified RVV-7, loss of 80% of PLA2 activity, the cytotoxic activity is unaffected | Daboia russelii russelii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Daboia russelii russelii |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Daboia russelii russelii | isoelectic focusing | - |
9.3 |
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